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Fig. 1-S1
Screen for epithelial markers that accumulate at sites of c4da dendrite contact.
( A) Summary of screen results. For a full list of markers screened, see Supplementary file 1. ( B) Co-localization of PLC?-PH-GFP and coracle. Maximum intensity projections of GFP, cora, and HRP (to label PNS neurons) immunostaining of larva expressing PLC?-PH-GFP in the epidermis. Bracket marks glial sheaths labeled by cora. ( C) PH domain mutation abrogates PLC?-PH-GFP sheath labeling. Image shows epidermal distribution of a PLC?-PH-GFP mutant (S39R) that is unable to bind PIP2 in a larva expressing the c4da-specific marker ppk-CD4-tdTomato. ( D) PIP2 is locally depleted from branch points. Plot depicts ratios of PLC?-PH-GFP signal intensity at branch points and on dendrite shafts adjacent to branchpoints. n = 20 branch points from five neurons (100 total). ( E) Co-localization of PLC?-PH-GFP and coracle. Maximum intensity projections of anti-GFP and anti-coracle immunostaining of larva expressing OSH2-GFP in the epidermis. ( F) Sheath localization of the septate junction protein Neurexin-IV. Maximum intensity projections of Neurexin-IV-GFP and ppkCD4-tdTomato are shown. Arrows mark sheaths labeled by Neurexin-IV-GFP.