ZFIN ID: ZDB-FIG-191230-929
Hughes et al., 2019 - Oligodendrocytes express synaptic proteins that modulate myelin sheath formation. Nature communications   10:4125 Full text @ Nat. Commun.
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5

Fig. 1

Axons accumulate synaptic vesicle release machinery under myelin sheaths. a Schematic of transgenes used to assess synaptic puncta density along myelinated and bare regions of axons. b Representative image of Syp-eGFP in a myelinated phox2b + axon in a living 3 dpf Tg(phox2b:GAL4; UAS:syp-eGFP; sox10:mRFP) larva. Bracketed dashed lines indicate two myelin sheaths (rectangles) on the axon. b′ Same as (b) for Vamp2-eGFP; animal genotype is Tg(phox2b:GAL4; sox10:mRFP) with transient expression of UAS:Vamp2-eGFP. c Representative images of Syp-eGFP distribution in myelinated phox2b + axons at 3, 4, and 5 dpf. d Comparisons of Syp-eGFP puncta density within bare vs myelinated regions of axons at each timepoint. n = 7 bare regions/98 puncta and 7 sheaths/18 puncta (3 dpf); 8 bare/119 puncta and 11 sheaths/43 puncta (4 dpf); 9 bare/75 puncta, and 12 sheaths/38 puncta (5 dpf). Wilcox rank-sum test, p = 0.13, p = 0.002, p = 0.00025 for 3, 4, and 5 dpf, respectively. Scale bars, 10 µm

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP co21Tg; uq1esTg; vu234Tg standard conditions Protruding-mouth reticulospinal neuron axon IFL
Day 4 reticulospinal neuron axon IFL
Day 5 reticulospinal neuron axon IFL
mRFP co21Tg; uq1esTg; vu234Tg standard conditions Protruding-mouth reticulospinal neuron myelin sheath IFL
Protruding-mouth reticulospinal neuron oligodendrocyte IFL
Day 4 reticulospinal neuron myelin sheath IFL
Day 4 reticulospinal neuron oligodendrocyte IFL
Day 5 reticulospinal neuron myelin sheath IFL
Day 5 reticulospinal neuron oligodendrocyte IFL
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.