IMAGE

Fig. 1

ID

ZDB-IMAGE-191230-945

Genes

GFP
mRFP

Publication

Hughes et al., 2019 - Oligodendrocytes express synaptic proteins that modulate myelin sheath formation

All Figures

Figures for Hughes et al., 2019

Image

Figure Caption

Fig. 1

Axons accumulate synaptic vesicle release machinery under myelin sheaths. a Schematic of transgenes used to assess synaptic puncta density along myelinated and bare regions of axons. b Representative image of Syp-eGFP in a myelinated phox2b + axon in a living 3 dpf Tg(phox2b:GAL4; UAS:syp-eGFP; sox10:mRFP) larva. Bracketed dashed lines indicate two myelin sheaths (rectangles) on the axon. b′ Same as (b) for Vamp2-eGFP; animal genotype is Tg(phox2b:GAL4; sox10:mRFP) with transient expression of UAS:Vamp2-eGFP. c Representative images of Syp-eGFP distribution in myelinated phox2b + axons at 3, 4, and 5 dpf. d Comparisons of Syp-eGFP puncta density within bare vs myelinated regions of axons at each timepoint. n = 7 bare regions/98 puncta and 7 sheaths/18 puncta (3 dpf); 8 bare/119 puncta and 11 sheaths/43 puncta (4 dpf); 9 bare/75 puncta, and 12 sheaths/38 puncta (5 dpf). Wilcox rank-sum test, p = 0.13, p = 0.002, p = 0.00025 for 3, 4, and 5 dpf, respectively. Scale bars, 10 µm

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.