ZFIN ID: ZDB-FIG-191230-770
Mano et al., 2019 - Brain-specific homeobox Bsx specifies identity of pineal gland between serially homologous photoreceptive organs in zebrafish. Communications biology   2:364 Full text @ Commun Biol
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 3

Effects of depletion of Bsx, Otx5 or Flh on the pineal gene expression at 48 hpf. ap Each panel represents head images of embryos (n = 12–32), all showing similar results. Embryos are viewed dorsally with anterior to the top. Arrowheads in (c), (m), and (p) indicate bsx expression in the parapineal organ. Scale bar, 30 μm

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bsx WT control Long-pec epiphysis ISH
Long-pec parapineal organ ISH
WT + MO1-bsx standard conditions Long-pec epiphysis ISH
WT + MO1-otx5 standard conditions Long-pec epiphysis ISH
WT + MO3-noto standard conditions Long-pec epiphysis ISH
exorh WT control Long-pec epiphysis ISH
WT + MO1-bsx standard conditions Long-pec epiphysis ISH
WT + MO1-otx5 standard conditions Long-pec epiphysis ISH
WT + MO3-noto standard conditions Long-pec Not Detected epiphysis ISH
otx5 WT control Long-pec epiphysis ISH
WT + MO1-bsx standard conditions Long-pec epiphysis ISH
pax6b WT control Long-pec epiphysis ISH
WT + MO1-bsx control Long-pec epiphysis ISH
rho WT control Long-pec Not Detected epiphysis ISH
WT + MO1-bsx standard conditions Long-pec epiphysis ISH
WT + MO1-otx5 standard conditions Long-pec Not Detected epiphysis ISH
WT + MO3-noto standard conditions Long-pec Not Detected epiphysis ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-bsx control Long-pec epiphysis pax6b expression decreased amount, abnormal
WT + MO1-bsx standard conditions Long-pec epiphysis exorh expression decreased amount, abnormal
Long-pec epiphysis otx5 expression decreased amount, abnormal
Long-pec epiphysis rho expression increased amount, abnormal
WT + MO1-otx5 standard conditions Long-pec epiphysis bsx expression decreased amount, abnormal
Long-pec epiphysis exorh expression decreased amount, abnormal
WT + MO3-noto standard conditions Long-pec epiphysis bsx expression decreased amount, abnormal
Long-pec epiphysis exorh expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Communications biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Commun Biol