FIGURE

Fig. 7

ID

ZDB-FIG-190812-20

Publication

Elworthy et al., 2019 - The role of endothelial cilia in post-embryonic vascular development

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Fig. 7

Endothelial cilia are present in the juvenile caudal fin vascular plexus. Immunofluorescence confocal slices of anti‐acetylated tubulin (red)‐ and anti‐GFP (green)‐stained caudal fin vascular plexus of 11‐dpf Tg(fli1a:LIFEACT‐clover)sh467^/+ (wt) (n = 15), 13‐dpf ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(fli1a:LIFEACT‐clover)sh467^/+ (ift54−/−; ubi:ift54) (n = 4), and 13‐dpf ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(kdrl:cre)s898^/+;Tg(fli1a:LIFEACT‐clover)sh467^/+ (ift54−/−;ubi:ift54;kdrl:cre) (n = 4). Slices at shallow and deep levels are shown along with orthogonal views resliced through the confocal stack at the level of cilia marked by arrows. Autofluorescent red blood cells (R) are visible in the vessel lumen. GFP, green fluorescent protein; WT, wild‐type. Scale bar = 10 μm

Expression Data

Gene:	mClover
Fish:	mitfa^w2/w2; traf3ip1^tp49d/tp49d; s898Tg/+; sh467Tg/+; sh488Tg/+ mitfa^w2/w2; traf3ip1^tp49d/tp49d; sh467Tg/+; sh488Tg/+
Anatomical Term:	caudal fin endothelial cell
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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