FIGURE

Fig. 8

ID

ZDB-FIG-190812-19

Publication

Elworthy et al., 2019 - The role of endothelial cilia in post-embryonic vascular development

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Fig. 8

Endothelial?specific excision of the Tg(ubi:loxP?ift54?loxP?myr?mcherry,myl7:EGFP)sh488?rescuing transgene causes no apparent further impairment to adult vasculature in ift54^tp49; Tg(ubi:loxP?ift54?loxP?myr?mcherry,myl7:EGFP)sh488 fish. A: Five?month?old ift54^tp49; Tg(ubi:loxP?ift54?loxP?myr?mcherry,myl7:EGFP)sh488^/+ (ift54−/−;ubi:ift54) (n = 24) and ift54^tp49; Tg(ubi:loxP?ift54?loxP?myr?mcherry,myl7:EGFP)sh488^/+; Tg(kdrl:cre)s898^/+ (ift54−/−;ubi:ift54;kdrl:cre) (n = 26) fish. The Tg(kdrl:cre)s898^/+ caused no apparent further impairment to fish with partial transgenic rescue of ift54^tp49. B: Confocal stack projections showing endothelial EGFP expression in flat?mount views of swim bladder posterior chamber, pectoral fin, and 4 day?postamputation regenerated caudal fin from sibling four?month?old Tg(ubi:loxP?ift54?loxP?myr?mcherry,myl7:EGFP)sh488^/+;Tg(fli1a:EGFP)y1^/+ (n = 5) (wt) and ift54^tp49; Tg(ubi:loxP?ift54?loxP?myr?mcherry,myl7:EGFP)sh488^/+; Tg(fli1a:EGFP)y1^/+ (representative of four animals analyzed) (ift54−/−;ubi:ift54) and ift54^tp49; Tg(ubi:loxP?ift54?loxP?myr?mcherry,myl7:EGFP)sh488^/+;Tg(kdrl:cre)s898^/+; Tg(fli1a:EGFP)y1^/+ (n = 5) (ift54−/−;ubi:ift54;kdrl:cre) fish. Yellow lines indicate approximate position of original fin amputation. EGFP, enhanced green fluorescent protein; WT, wild?type. Scale bars = 300 μm

Expression Data

Gene:	EGFP
Fish:	mitfa^w2/w2; traf3ip1^tp49d/tp49d; s898Tg/+; sh488Tg/+; y1Tg/+ mitfa^w2/w2; traf3ip1^tp49d/tp49d; sh488Tg/+; y1Tg/+
Condition:	amputation: caudal fin
Anatomical Terms:	pectoral fin endothelial cell regenerating fin endothelial cell swim bladder endothelial cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^w2/w2; traf3ip1^tp49d/tp49d; sh467Tg/+; sh488Tg/+ mitfa^w2/w2; traf3ip1^tp49d/tp49d; sh488Tg
Observed In:	vertebral column
Stage:	Adult

Phenotype Detail

Acknowledgments

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