FIGURE

Fig. 6

ID

ZDB-FIG-190812-22

Publication

Elworthy et al., 2019 - The role of endothelial cilia in post-embryonic vascular development

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Fig. 6

Endothelial‐specific excision of the Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488transgene causes no apparent further impairment to secondary caudal fin vasculature in ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 fish. A: SPIM stack projections of endothelial cells marked with Clover expression in the caudal fin of 10‐dpf and 14‐dpf Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(kdrl:cre)s898^/+;Tg(fli1a:LIFEACT‐clover)sh467^/+ fish, showing endothelial‐specific Clover and myr‐mcherry expression. B: SPIM stack projections of endothelial cells marked with Clover in the caudal fin of 14‐dpf ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(fli1a:LIFEACT‐clover)sh467^/+ (ift54−/−; ubi:ift54) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(kdrl:cre)s898^/+;Tg(fli1a:LIFEACT‐clover)sh467^/+ (ift54−/−; ubi:ift54; kdrl:cre). The secondary caudal fin vasculature forms in the absence of endothelial ift54 function. C: SPIM stack projections of endothelial cells marked with Clover in the caudal fin of 13‐dpf ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(fli1a:LIFEACT‐clover)sh467^/+ (ift54−/−; ubi:ift54) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(kdrl:cre)s898^/+;Tg(fli1a:LIFEACT‐clover)sh467^/+ (ift54−/−; ubi:ift54; kdrl:cre) fish. The Tg(kdrl:cre)s898^/+ caused no apparent impairment to the developing fin vascular bed in the fish with partial transgenic rescue of ift54^tp49. Chart shows caudal fin vessel bed relative areas measured from SPIM images. Overlay shows median and interquartile range. Two‐tailed Mann‐Whitney test P = 0.7223

Expression Data

Genes:	mCherry mClover
Fish:	mitfa^w2/w2; s898Tg/+; sh467Tg/+; sh488Tg/+ mitfa^w2/w2; traf3ip1^tp49d/tp49d; s898Tg/+; sh467Tg/+; sh488Tg/+ mitfa^w2/w2; traf3ip1^tp49d/tp49d; sh467Tg/+; sh488Tg/+
Anatomical Term:	caudal fin endothelial cell
Stage Range:	Days 7-13 to Days 14-20

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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