FIGURE

Fig. 12

ID

ZDB-FIG-190812-15

Publication

Elworthy et al., 2019 - The role of endothelial cilia in post-embryonic vascular development

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Fig. 12

Endothelial‐specific excision of the Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488‐rescuing transgene causes no apparent further impairment to perivascular mural cells in the caudal fin vascular plexus of ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 fish. A: Confocal stack projections showing mcherry fluorescence from 17‐dpf caudal fins of ift54^tp4; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+; Tg(tagln:mcherry)sh441^/+ (ift54−/−; ubi:ift54; tagln:mcherry) and ift54^tp4; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(kdrl:cre)s898; Tg(tagln:mcherry)sh441^/+ (ift54−/−; ubi:ift54; tagln:mcherry; kdrl:cre). Also shown are binary threshold processed images as used for quantification of the area of Tg(tagln:mcherry)sh441^/+ expression. Single‐focal‐plane transmitted light views are shown for orientation. B: Chart shows % of field of view with >threshold mcherry expression as measured from processed confocal projections of caudal fins. Overlay shows median and interquartile range. Two‐tailed Mann‐Whitney test P = 0.5362. The Tg(kdrl:cre)s898^/+ caused no apparent impairment to mural cell coverage in the newly developed fin vascular bed in the fish with partial transgenic rescue of ift54^tp49

Expression Data

Gene:	mCherry
Fish:	mitfa^w2/w2; traf3ip1^tp49d/tp49d; s898Tg/+; sh441Tg/+; sh488Tg/+ mitfa^w2/w2; traf3ip1^tp49d/tp49d; sh441Tg/+; sh488Tg/+
Anatomical Term:	caudal fin vascular smooth muscle
Stage:	Days 14-20

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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