FIGURE

Fig. S10

ID

ZDB-FIG-190809-8

Publication

Song et al., 2019 - HDAC1-mediated repression of the retinoic acid-responsive gene ripply3 promotes second heart field development

Other Figures

All Figure Page

Back to All Figure Page

Fig. S10

Generation of ripply3 mutants.

(A) Schematic of the ripply3 exons and the gRNA targeting exon of the ripply3 gene. (B) The ripply3 mutant allele used deletes 61bp including the start codon. (C,D) WT sibling and ripply3mutants at 48 hpf. (E,F) Ripply3 transcripts are not detectable in ripply3 mutants at 30 hpf. Arrows indicate posterior pharyngeal region where ripply3 is expressed in WT embryos. Views are dorsal with anterior up. (G) RT-qPCR for ripply3 in ripply3 mutants at 36 hpf indicates the transcripts are essentially undetectable. Primers used do not bind within the deleted region. (H) Quantification of atrial CMs in crg^wt+het; ripply3^wt+het, crg^wt+het; ripply3^-/-, crg^-/-; ripply3^wt+het, and crg^-/-; ripply3^-/- embryos at 48 hpf (n = 37 for crg^wt+het; ripply3^wt+het, n = 10 for crg^wt+het; ripply3^-/-, n = 48 for crg^-/-; ripply3^wt+het, n = 18 for crg^-/-; ripply3^-/-).

Expression Data

Gene:	ripply3
Fish:	WT ripply3^{ci1010/ci1010}
Anatomical Terms:	pharyngeal arch anatomical region whole organism
Stage Range:	Prim-15 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ripply3^{ci1010/ci1010}
Observed In:	pharyngeal arch anatomical region whole organism
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.