Fig 8

(A,B) Control and transgenic hsp70l:GFP-ripply3 embryos at 48 hpf following heat-shock at 20 hpf. (C,D) Hearts from control and hsp70l:GFP-ripply3 transgenic embryos with the myl7:DsRed2-NLS transgene at 48 hpf following heat-shock at 20 hpf. Purple alone indicates ventricle. Yellow indicates atrium. Arrows indicate arterial pole of the ventricle. (E) Quantification of CMs in control and hsp70l:GFP-ripply3 transgenic embryos at 48 hpf following GFP-ripply3 induction at 20 hpf (n = 18 for control, n = 29 for GFP-ripply3⁺). (F) Quantification of VCs in crg^wt+het; ripply3^wt+het, crg^wt+het; ripply3^-/-, crg^-/-; ripply3^wt+het, and crg^-/-; ripply3^-/- embryos at 48 hpf (n = 37 for crg^wt+het; ripply3^wt+het, n = 10 for crg^wt+het; ripply3^-/-, n = 48 for crg^-/-; ripply3^wt+het, n = 18 for crg^-/-; ripply3^-/-). (G) Quantification of VCs in WT (crg^wt+het—uninjected) sibling, crg^wt+het–Tbx1 depleted, crg^-/-—uninjected, and crg^-/-—Tbx1-depleted embryos at 48 hpf (n = 11 for crg^wt+het—control uninjected, n = 10 for crg^wt+het–Tbx1-depleted, n = 10 for crg^-/-—control uninjected, n = 10 for crg^-/-—Tbx1-depleted). For all CM quantification, embryos contained the myl7:DsRed2-NLS transgene.