FIGURE

Fig 3

ID

ZDB-FIG-190723-793

Publication

Song et al., 2019 - HDAC1-mediated repression of the retinoic acid-responsive gene ripply3 promotes second heart field development

Other Figures

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Fig 3

SHF-derived VC and smooth muscle development is impaired in the <italic>crg</italic> mutants.

(A-B”) Representative images of hearts from photoconverted WT sibling and crg mutant myl7:NLS-KikGR embryos at 48 hpf. The arterial poles (brackets) are to the right. (C) Quantification of later-differentiating VCs (Yellow⁺/Purple^- cells) (n = 10 for WT and crg mutants). (D,E) Confocal images of IHC for MHC and Elnb in WT and crg mutant embryos at 72 hpf. n = 10 WT and n = 10 crg mutants embryos examined. (F,G) Confocal images of DAF-2DA staining coupled with IHC for Vmhc in WT sibling and crg mutant embryos at 96 hpf. n = 10 WT and n = 10 crg mutants embryos examined. Images in D-G are frontal views with anterior up. Arrows indicate Elnb and DAF-2DA staining of the bulbous arteriosus.

Expression Data

Gene:	KikGR
Antibodies:	Ab-MF20 Ab2-elnb Ab3-vmhc
Fish:	WT hdac1^nl18/nl18 hsc6Tg hdac1^nl18/nl18; hsc6Tg
Anatomical Terms:	bulbus arteriosus bulbus arteriosus smooth muscle cardiac muscle cell cardiac ventricle skeletal muscle
Stage Range:	Long-pec to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hdac1^nl18/nl18 hdac1^nl18/nl18; hsc6Tg
Observed In:	bulbus arteriosus bulbus arteriosus smooth muscle cardiac ventricle cardiac muscle cell secondary heart field specification smooth muscle
Stage Range:	Long-pec to Day 4

Phenotype Detail

Acknowledgments

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