FIGURE

Fig. S9

ID

ZDB-FIG-190809-7

Publication

Song et al., 2019 - HDAC1-mediated repression of the retinoic acid-responsive gene ripply3 promotes second heart field development

Other Figures

All Figure Page

Back to All Figure Page

Fig. S9

Generation and validation of the heat-shock inducible GFP-ripply3 transgenic line.

(A) Schematic of the heat-shock inducible hsp70l:GFP-ripply3 transgene. (B-E) Heat-shock induction of GFP-ripply3 at 24 hpf. Control embryos are heat-shocked non-transgenic siblings. (F,H) Control and hsp70l:GFP-ripply3 embryos at 48 hpf following heat-shock at 10 hpf. Lateral views with anterior to the left. (G,I) Hearts from control and hsp70l:GFP-ripply3; myl7:NLS-DsRed2 embryos at 48 hpf following heat-shock at 10 hpf. Frontal views. Purple alone indicates ventricle. Yellow indicates atrium. Arrows indicate arterial pole of the ventricle. (J) Quantification of CMs from control and hsp70l:GFP-ripply3; myl7:NLS-DsRed2 embryos at 48 hpf following heat-shock at 10 hpf (n = 25 for control and GFP-ripply3⁺). (K,M) Control and ripply3 mRNA-injected embryos at 48 hpf. Lateral views with anterior to the left. (L,N) Hearts from control and ripply3 mRNA-injected myl7:NLS-DsRed2 embryos at 48 hpf. Frontal views. Purple alone indicates ventricle. Yellow indicates atrium. Arrows indicate arterial pole of the ventricle. (O) Quantification of CMs from control and ripply3 mRNA-injected myl7:NLS-DsRed2 embryos at 48 hpf (n = 21 for control and ripply3 mRNA-injected embryos).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.