ZFIN Image: Song et al., 2019, Fig. S10

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Figure Caption

Fig. S10

Generation of ripply3 mutants.

(A) Schematic of the ripply3 exons and the gRNA targeting exon of the ripply3 gene. (B) The ripply3 mutant allele used deletes 61bp including the start codon. (C,D) WT sibling and ripply3mutants at 48 hpf. (E,F) Ripply3 transcripts are not detectable in ripply3 mutants at 30 hpf. Arrows indicate posterior pharyngeal region where ripply3 is expressed in WT embryos. Views are dorsal with anterior up. (G) RT-qPCR for ripply3 in ripply3 mutants at 36 hpf indicates the transcripts are essentially undetectable. Primers used do not bind within the deleted region. (H) Quantification of atrial CMs in crg^wt+het; ripply3^wt+het, crg^wt+het; ripply3^-/-, crg^-/-; ripply3^wt+het, and crg^-/-; ripply3^-/- embryos at 48 hpf (n = 37 for crg^wt+het; ripply3^wt+het, n = 10 for crg^wt+het; ripply3^-/-, n = 48 for crg^-/-; ripply3^wt+het, n = 18 for crg^-/-; ripply3^-/-).

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Expression / Labeling details

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Acknowledgments

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