Fig. 1

Villani et al., 2019 - Clearance by Microglia Depends on Packaging of Phagosomes into a Unique Cellular Compartment
Other Figures
All Figure Page
Back to All Figure Page
Fig. 1

Characterization of bubblebrain, a Mutant with Bloated Microglia

(A and B) Neutral red staining in a 4 dpf wild-type (A) and blbNY007 (B) embryos.

(C and D) Microglia labeled with Tg(Apo-E:lynGFP) in a 4 dpf wild-type (C) and blbNY007 (D) embryo.

(E and F) Microglia cell labeled with Tg(spi1b:Gal4,UAS:GFP) in a 4 dpf wild-type (E) and blbNY007 (F) embryo. Scale bar, 10 μm. 

(G) Quantification of the “bubble” size in blbNY007 embryos from 2.5 to 4.5 dpf in optic tectum (OT, red plots) and in the hindbrain (HB, orange plots). Whiskers: 5th/95th percentile, p value < 0.0001.

(H) Quantification of apoptotic neurons visualized by Acridine Orange in wild-type (n = 20), blbNY007 (n = 17) and in wild-type (n = 18) and blbNY007 (n= 19) injected with pU1 morpholino to deplete microglia in 3.5 dpf embryos; representative images in (J) and (K). Whiskers: 5th/95th percentile, p value < 0.0001.

(I) Quantifications of phagocytic events per cell per hour in wild-type (n = 50; n = 47) and blbNY007 (n = 61; n = 77) embryos at 3.5 dpf and 4 dpf, respectively. Whiskers: 5th/95th percentile, p value < 0.0001.

(J and K) Dorsal view of a 3.5 dpf wild-type (J) and blbNY007 (K) embryo injected with pU1 morpholino to deplete microglia and stained with Acridine orange to visualize apoptotic neurons; quantifications in (H).

(L) Microglia cell labeled with Tg(mpeg:GFP-caax) and Tg(NBT:secA5-BFP) to visualize apoptotic neurons in a 4 dpf blbNY007 embryo.

Expression Data
Anatomical Terms:
Stage: Day 4

Expression Detail
Antibody Labeling
Phenotype Data
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 4

Phenotype Detail
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 49(1), Villani, A., Benjaminsen, J., Moritz, C., Henke, K., Hartmann, J., Norlin, N., Richter, K., Schieber, N.L., Franke, T., Schwab, Y., Peri, F., Clearance by Microglia Depends on Packaging of Phagosomes into a Unique Cellular Compartment, 77-88.e7, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell