ZFIN ID: ZDB-FIG-191224-6
Villani et al., 2019 - Clearance by Microglia Depends on Packaging of Phagosomes into a Unique Cellular Compartment. Developmental Cell   49(1):77-88.e7 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 7 Gastrosomal Defects Affect Microglial Functionality

(A) 4 dpf wild-type Tg(mpeg:GFP-caax) microglia stained with Nile Blue (cyan) in an embryo treated with Camptothecin to increase apoptosis. Scale bar, 5 μm. The white dotted line marks the gastrosome.

(B) SPIM time-lapse of representative 4 dpf wild-type Tg(mpeg:GFP-caax) microglia stained with Nile Blue (cyan) in an embryo treated with Camptothecin to increase apoptosis. Scale bar, 10 μm. The white dotted line marks the shrinking gastrosome. See also Video S8.

(C) Quantification of microglia responding to an injury in wild-type and blbNY007 microglia at different developmental stages; wt, 3.5 dpf, n = 20; blbNY007, 3.5 dpf, n = 20; wt, 4 dpf, n = 20; and blbNY007, 4 dpf, n = 16; whiskers: 5th/95th percentile, p value < 0.0001. See also Video S9 (left, wild-type embryo and right, blb embryo).

(D) Quantification of engulfment duration in wild-type (n = 20) and blbNY007 microglia (n = 11), whiskers: 5th/95th percentile, p value < 0.001.

(E) RAW264.7 macrophages unfed (left column) or fed with HK H2B mCherry cells and fixed 24 h after apoptosis induction (right column). Slc37a2 antibody staining in untreated controls (upper panels) and in cells transfected with an siRNA targeting Slc37a2.

(F) Quantification of the gastrosomal size (μm) in RAW macrophages fed with apoptotic HeLa cells in the absence or in the presence of Slc37a2 siRNA, p value < 0.0001. Representative images of this experiment in Figure S3A (yellow arrowheads).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zh901Tg chemical treatment by environment: camptothecin Day 4 microglial cell phagocytic vesicle IFL
Day 4 microglial cell plasma membrane IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
slc37a2t30301/t30301 standard conditions Protruding-mouth microglial cell microglial cell migration decreased occurrence, abnormal
Day 4 microglial cell microglial cell migration decreased occurrence, abnormal
Day 4 microglial cell phagocytosis, engulfment increased duration, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 49(1), Villani, A., Benjaminsen, J., Moritz, C., Henke, K., Hartmann, J., Norlin, N., Richter, K., Schieber, N.L., Franke, T., Schwab, Y., Peri, F., Clearance by Microglia Depends on Packaging of Phagosomes into a Unique Cellular Compartment, 77-88.e7, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell