ZFIN ID: ZDB-FIG-190723-257
Iwasaki et al., 2019 - Essential role of prostaglandin E2 and the EP3 receptor in lymphatic vessel development during zebrafish embryogenesis. Scientific Reports   9:7650 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Long-pec

Figure 6

Effect of EP3 receptor deficiency on lymphatic specification and development. (A) Sequence alignment of the wild-type and mutant EP3 receptor. The seven base-pair deletion in mutant EP3 receptor is indicated by a dotted line. Transmembrane (TM) regions of the wild-type EP3 receptor are indicated by gray boxes. (B) Number of live births of EP3+/+, EP3+/−, and EP3−/− zebrafish. (C) HeLa cells were transfected with an expression vector encoding either the wild-type or mutant EP3 receptor. After 24 h, cells were incubated with loading buffer for 1 h and then treated with Veh or Sulp. Induced intracellular Ca2+ mobilization was evaluated by AUC analysis. *P < 0.05, **P < 0.01 vs Veh. Each value represents the mean ± SEM (N = 3). (D) Relative expression levels of lyve1b were quantified by RT-qPCR in EP3+/+, EP3+/−, and EP3−/− zebrafishs at 24 hpf. Values are shown relative to the value obtained with EP3+/+. Each value represents the mean ± SEM (N = 8–12) *P < 0.05 vs EP3+/+. (E) Expression of lyve1b was analyzed by WISH in EP3+/+, EP3+/−, and EP3−/− zebrafish at 36 hpf. (F) Expression of lyve1b was analyzed by WISH in EP3+/+ and EP3−/− zebrafish at 52 hpf. The PL is indicated by an arrowhead. The number at the bottom right of each panel indicates the number of embryos demonstrating the phenotype shown in the panel over the total number of embryos analyzed in a representative experiment.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
lyve1b WT standard conditions Prim-5 lymph vessel ISH
Long-pec horizontal myoseptum ISH
ptger3kum1/+ standard conditions Prim-5 lymph vessel ISH
ptger3kum1/kum1 standard conditions Prim-5 lymph vessel ISH
Long-pec Not Detected horizontal myoseptum ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ptger3kum1/kum1 standard conditions Prim-5 lymph vessel lyve1b expression decreased distribution, abnormal
Long-pec horizontal myoseptum lyve1b expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.