ZFIN ID: ZDB-FIG-190723-254
Iwasaki et al., 2019 - Essential role of prostaglandin E2 and the EP3 receptor in lymphatic vessel development during zebrafish embryogenesis. Scientific Reports   9:7650 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Prim-25

Figure 2

Role of the PGE2-EP3 pathway in the lymphatic specification from venous to lymphatic endothelial cells. (A) Tg(fli1a:egfp) embryos were treated with Veh or Indo (100 μM) for the indicated times. The ratio of PL-positive segments at 60 hpf was quantified. Each value represents the mean ± SEM (N = 5). **P < 0.01 vs Veh. (B) Relative expression levels of lyve1b were quantified by RT-qPCR in morphants at 24 and 36 hpf. Values are shown relative to the value obtained with Cont MO at 24 hpf. Each value represents the mean ± SEM (N = 3–4) *P < 0.05, **P < 0.01 vs Cont MO at each corresponding time. (C,D) Expression of lyve1b was analyzed by WISH in morphants at 24 hpf (C) and 36 hpf (D). (E,F) Zebrafish embryos were treated with Veh or Indo (100 μM) in the absence or presence of EP agonists (10 μM) from 0 to 24 hpf. The expression level of lyve1b was quantified by RT-qPCR (E). The values are shown relative to the value obtained with Veh. Each value represents the mean ± SEM (N = 3–4). Expression of lyve1b was analyzed by WISH at 24 hpf (F). The number at the bottom right of each panel indicates the number of embryos demonstrating the phenotype shown in the panel over the total number of embryos analyzed in a representative experiment.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
lyve1b WT control Prim-5 whole organism RTPCR
WT standard conditions Prim-5 lymph vessel ISH
Prim-25 lymph vessel ISH
WT chemical treatment: indometacin Prim-5 lymph vessel ISH
Prim-5 whole organism RTPCR
WT chemical treatment: indometacin, chemical treatment: sulprostone Prim-5 lymph vessel ISH
Prim-5 whole organism RTPCR
Prim-5 whole organism ISH
y1Tg + MO1-ptger3 standard conditions Prim-5 lymph vessel ISH
Prim-25 lymph vessel ISH
y1Tg + MO2-ptger3 standard conditions Prim-5 lymph vessel ISH
Prim-25 lymph vessel ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: indometacin Prim-5 lymph vessel lyve1b expression decreased distribution, abnormal
Prim-5 whole organism lyve1b expression decreased amount, abnormal
y1Tg + MO1-ptger3 standard conditions Prim-5 lymph vessel lyve1b expression decreased distribution, abnormal
Prim-25 lymph vessel lyve1b expression decreased distribution, abnormal
y1Tg + MO2-ptger3 standard conditions Prim-5 lymph vessel lyve1b expression decreased distribution, abnormal
Prim-25 lymph vessel lyve1b expression decreased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.