ZFIN ID: ZDB-FIG-190611-53
Tu et al., 2018 - Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis. Nature communications   9:5277 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 10-13 somites to Protruding-mouth

Fig. 1

FSD1 is required for ciliogenesis in human cells and zebrafish. a RPE-1 cells were transfected with the indicated siRNA and serum-starved for 48 h. Cells were stained with the indicated antibodies and examined by immunofluorescence microscopy. Scale bar, 5 μm. bEffects of FSD1 depletion on cilia formation in cycling (+Serum) or quiescent (−Serum) RPE-1 cells. Acetylated α-tubulin (Ac-tubulin) is ciliary marker. c Cilia defects induced by FSD1 knockdown in RPE-1 cells were rescued by expressing a GFP-tagged, RNAi-resistant form of FSD1. Data are presented as mean ± s.d. of three independent experiments. n number of cells. de Fsd1 morphants (aMO and sMO) displayed curved body and pericardial edema at 72 hpf. The arrows mark curved body and arrowheads mark pericardial edema. The fsd1-misMO were used as control. Scale bars, 1 mm. Data are presented as the mean ± s.d. of three independent experiments. fg Fsd1 MOs (aMO and sMO) caused left-right asymmetry defects. The spaw probe was used to label the left lateral plate mesoderm in the whole-mount in situ hybridization at 18-somite stage. Scale bar, 150 μm. n number of fishes. hjFsd1 MOs (aMO and sMO) impaired ciliogenesis in Kupffer’s vesicle at 10-somite stage (10 s). Bars indicate the median. Scale bar, 10 μm. k Fsd1 knockout impaired ciliogenesis in Kupffer’s vesicle at 10 s. Bars indicate the median. Scale bar, 10 μm. In all panels, statistical comparisons between two groups were carried out by two-tailed t-test. *P < 0.05, **P < 0.01, ***P < 0.001

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
spaw AB control 14-19 somites lateral plate mesoderm left side ISH
AB + MO1-fsd1 control 14-19 somites lateral plate mesoderm left side ISH
14-19 somites lateral plate mesoderm right side ISH
AB + MO2-fsd1 control 14-19 somites lateral plate mesoderm left side ISH
14-19 somites lateral plate mesoderm right side ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + CRISPR1-fsd1 standard conditions 10-13 somites Kupffer's vesicle cilium decreased amount, abnormal
AB + MO1-fsd1 control 14-19 somites lateral plate mesoderm right side spaw expression mislocalised, abnormal
AB + MO1-fsd1 standard conditions 10-13 somites Kupffer's vesicle cilium decreased amount, abnormal
10-13 somites Kupffer's vesicle cilium decreased length, abnormal
14-19 somites determination of left/right symmetry process quality, abnormal
Protruding-mouth pericardium edematous, abnormal
Protruding-mouth whole organism increased curvature, abnormal
AB + MO2-fsd1 control 14-19 somites lateral plate mesoderm right side spaw expression mislocalised, abnormal
AB + MO2-fsd1 standard conditions 10-13 somites Kupffer's vesicle cilium decreased amount, abnormal
10-13 somites Kupffer's vesicle cilium decreased length, abnormal
14-19 somites determination of left/right symmetry process quality, abnormal
Protruding-mouth pericardium edematous, abnormal
Protruding-mouth whole organism increased curvature, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.