ZFIN ID: ZDB-FIG-190611-56
Tu et al., 2018 - Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis. Nature communications   9:5277 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. S3

(a) Fsd1 knock out efficiency was validated by sequencing. (b) Fsd1 knock out displayed curved body at 72 hpf. The arrow marked curved body. Scale bars, 1 mm. (c) Fsd1 knock out caused left-right asymmetry defects. The cmlc probe was used to label the heart tube in the whole-mount in situ hybridization at 26 hpf. Scale bar, 100 μm. n, number of fishes.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myl7 AB control Prim-5 heart tube ISH
AB + CRISPR1-fsd1 standard conditions Prim-5 heart tube ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + CRISPR1-fsd1 standard conditions Prim-5 determination of heart left/right asymmetry process quality, abnormal
Prim-5 heart tube mislocalised, abnormal
Protruding-mouth whole organism increased curvature, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.