ZFIN ID: ZDB-FIG-190611-56
Tu et al., 2018 - Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis. Nature communications   9:5277 Full text @ Nat. Commun.
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. S3

(a) Fsd1 knock out efficiency was validated by sequencing. (b) Fsd1 knock out displayed curved body at 72 hpf. The arrow marked curved body. Scale bars, 1 mm. (c) Fsd1 knock out caused left-right asymmetry defects. The cmlc probe was used to label the heart tube in the whole-mount in situ hybridization at 26 hpf. Scale bar, 100 μm. n, number of fishes.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myl7 AB control Prim-5 heart tube ISH
AB + CRISPR1-fsd1 standard conditions Prim-5 heart tube ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + CRISPR1-fsd1 standard conditions Prim-5 determination of heart left/right asymmetry process quality, abnormal
Prim-5 heart tube mislocalised, abnormal
Protruding-mouth whole organism increased curvature, abnormal
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.