ZFIN ID: ZDB-FIG-181121-2
Sanchez et al., 2018 - Quinoxaline protects zebrafish lateral line hair cells from cisplatin and aminoglycosides damage. Scientific Reports   8:15119 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Conditions:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Day 5

Fig. 2

Dose protection curve against CP. 5dpf Tg(brn3c:GFP) larvae were incubated with 50 µM to 800 µM of CP (A,C,E,G) for 6 hours or pre-treated with 300 µM of Qx for 2 hours and then co-treated with Qx and CP (50µM-800µM) for 6 hours (B,D,F,H). Animals were fixed and immunostained for GFP (green) and otoferlin (red). Control animals were exposed to vehicle alone (DMSO). (I) Quantification of the number of hair cells per neuromast after the different treatments represented as mean +/− SEM. One-way ANOVA, Dunnett post test. ***p < 0.001. Black asterisks compared versus DMSO-treated animals. Red asterisks compared versus the corresponding CP concentration. (J) Scores for neuromast morphology (see Materials and Methods). Scale bar: 6 μm. Data were taken from at least 20 animals and 3 experiments runs.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP s273tTg chemical treatment by environment: cisplatin Day 5 neuromast hair cell plasma membrane IHC
s273tTg chemical treatment by environment: cisplatin, chemical treatment by environment: quinoxaline Day 5 neuromast hair cell plasma membrane IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-otof IHC s273tTg chemical treatment by environment: cisplatin Day 5 neuromast hair cell
IHC s273tTg chemical treatment by environment: cisplatin, chemical treatment by environment: quinoxaline Day 5 neuromast hair cell
Phenotype Details
Fish Conditions Stage Phenotype
s273tTg chemical treatment by environment: cisplatin Day 5 neuromast has fewer parts of type neuromast hair cell, abnormal
Day 5 neuromast malformed, abnormal
s273tTg chemical treatment by environment: cisplatin, chemical treatment by environment: quinoxaline Day 5 neuromast has normal numbers of parts of type neuromast hair cell, ameliorated
Day 5 neuromast has number of neuromast hair cell, ameliorated
Day 5 neuromast morphology, ameliorated
Acknowledgments:
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