ZFIN ID: ZDB-FIG-181121-6
Sanchez et al., 2018 - Quinoxaline protects zebrafish lateral line hair cells from cisplatin and aminoglycosides damage. Scientific Reports   8:15119 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Day 5

Fig. 6

Qx protects against ototoxin-induced HC death and promotes supporting cell proliferation. (AI) TUNEL assay (red) was performed in zebrafish incubated with vehicle or 300 µM of Qx alone (controls) or with the corresponding ototoxin with or without Qx 300 µM. Animals were counterstained with phalloidin (green). (A) Neomycin (Neo) 200 µM incubation for 30 min. (B) Gentamicin (GM) 50 µM incubation for 1 hour. (C) Cisplatin (CP) 400 µM incubation for 2 hours. (D) GM 50 µM incubation for 1 hour followed by recovery for 5 hours. (E) Incubation with Qx 300 µM for 8 hours and Neo 200 µM for 30 min. (F) Incubation with Qx 300 µM for 8 hours and GM 50 µM for 1 hour. (G) Qx 300 µM for 8 hours + CP 400 µM for 2 hours. (H) Qx 300 µM incubation for 8 hours + GM 50 µM for 1 hour followed by 5 hours recovery. Asterisks denote TUNEL-positive HCs. (I) The percentage of TUNEL-positive neuromasts was calculated for each treatment and represented as mean +/− SEM. (JR) Proliferation assays were performed in 5dpf Tg(brn3c:GFP) in the presence/absence of Qx and the corresponding ototoxin, by the BrdU-labelling method (red). Animals were immunostained for GFP (green). (J) control. (K) Neo 200 µM 30 min. (L) GM 50 µM 1 hour. (M) CP: 400 µM 2 hours. (N) Qx 300 µM 8 hours. (O) Qx 300 µM 8 hours + Neo 200 µM 30 min. (P) Qx 300 µM 8 hours + GM 50 µM 1 hour. (Q) Qx 300 µM 8 hours + CP 400 µM 2 hours. Asterisks denote neuromast supporting cells positive for BrdU. (R) The percentage of BrdU-positive supporting cells per neuromast was calculated for each treatment and represented as mean +/− SEM. One-way ANOVA, *p < 0.05, **p < 0.01. Black asterisks compared versus corresponding control. Red asterisk compared versus the corresponding ototoxin-only treatment. Scale bar: (AH) 10 µm, (JO) 7 μm. Data were taken from at least 15 animals and 3 experiments runs.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP s273tTg control Day 5 neuromast hair cell plasma membrane IHC
s273tTg chemical treatment by environment: cisplatin Day 5 neuromast hair cell plasma membrane IHC
s273tTg chemical treatment by environment: gentamycin Day 5 neuromast hair cell plasma membrane IHC
s273tTg chemical treatment by environment: neomycin Day 5 neuromast hair cell plasma membrane IHC
s273tTg chemical treatment by environment: quinoxaline Day 5 neuromast hair cell plasma membrane IHC
s273tTg chemical treatment by environment: cisplatin, chemical treatment by environment: quinoxaline Day 5 neuromast hair cell plasma membrane IHC
s273tTg chemical treatment by environment: gentamycin, chemical treatment by environment: quinoxaline Day 5 neuromast hair cell plasma membrane IHC
s273tTg chemical treatment by environment: neomycin, chemical treatment by environment: quinoxaline Day 5 neuromast hair cell plasma membrane IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB chemical treatment by environment: cisplatin Day 5 neuromast hair cell apoptotic process increased occurrence, abnormal
AB chemical treatment by environment: gentamycin Day 5 neuromast hair cell apoptotic process increased occurrence, abnormal
AB chemical treatment by environment: neomycin Day 5 neuromast hair cell apoptotic process increased occurrence, abnormal
AB chemical treatment by environment: cisplatin, chemical treatment by environment: quinoxaline Day 5 neuromast hair cell apoptotic process normal occurrence, ameliorated
AB chemical treatment by environment: gentamycin, chemical treatment by environment: quinoxaline Day 5 neuromast hair cell apoptotic process increased occurrence, ameliorated
AB chemical treatment by environment: neomycin, chemical treatment by environment: quinoxaline Day 5 neuromast hair cell apoptotic process increased occurrence, ameliorated
s273tTg chemical treatment by environment: cisplatin Day 5 neuromast support cell mitotic cell cycle occurrence, normal
s273tTg chemical treatment by environment: gentamycin Day 5 neuromast support cell mitotic cell cycle occurrence, normal
s273tTg chemical treatment by environment: neomycin Day 5 neuromast support cell mitotic cell cycle occurrence, normal
s273tTg chemical treatment by environment: quinoxaline Day 5 neuromast support cell mitotic cell cycle increased occurrence, abnormal
s273tTg chemical treatment by environment: cisplatin, chemical treatment by environment: quinoxaline Day 5 neuromast support cell mitotic cell cycle increased occurrence, abnormal
s273tTg chemical treatment by environment: gentamycin, chemical treatment by environment: quinoxaline Day 5 neuromast support cell mitotic cell cycle increased occurrence, abnormal
s273tTg chemical treatment by environment: neomycin, chemical treatment by environment: quinoxaline Day 5 neuromast support cell mitotic cell cycle increased occurrence, abnormal
Acknowledgments:
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