ZFIN ID: ZDB-FIG-181121-1
Sanchez et al., 2018 - Quinoxaline protects zebrafish lateral line hair cells from cisplatin and aminoglycosides damage. Scientific Reports   8:15119 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Conditions:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Day 5

Fig. 1

Qx protects against cisplatin ototoxicity. 5dpf Tg(brn3c:GFP) larvae were incubated with vehicle alone (DMSO, A), 400 μM of cisplatin (CP, B), pre-treated with Qx for 2 hours and then incubated with CP for 6 hours (Qx, CP, C,E,G) or pre-treated with Qx for 2 hours and then co-treated with Qx and CP for 6 more hours (Qx + CP, D,F,H). Animals were fixed and immunostained for GFP (green) and otoferlin (red). (I) Quantification of the number of hair cells per neuromast after the different treatments represented as mean +/− SEM. One-way ANOVA, Dunnett post test. *p < 0.05, ***p < 0.001. Black asterisks compared versus control. Red asterisks compared versus CP 400 µM. (J) Scores for neuromast morphology (see Materials and Methods). Scale bar: 6 μm. Data were taken from at least 20 animals and 3 experiments runs.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP s273tTg control Day 5 neuromast hair cell plasma membrane IHC
s273tTg chemical treatment by environment: cisplatin Day 5 neuromast hair cell plasma membrane IHC
s273tTg chemical treatment by environment: cisplatin, chemical treatment by environment: quinoxaline Day 5 neuromast hair cell plasma membrane IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-otof IHC s273tTg control Day 5 neuromast hair cell
IHC s273tTg chemical treatment by environment: cisplatin Day 5 neuromast hair cell
IHC s273tTg chemical treatment by environment: cisplatin, chemical treatment by environment: quinoxaline Day 5 neuromast hair cell
Phenotype Details
Fish Conditions Stage Phenotype
s273tTg chemical treatment by environment: cisplatin Day 5 neuromast has fewer parts of type neuromast hair cell, abnormal
Day 5 neuromast malformed, abnormal
s273tTg chemical treatment by environment: cisplatin, chemical treatment by environment: quinoxaline Day 5 neuromast has normal numbers of parts of type neuromast hair cell, ameliorated
Day 5 neuromast has number of neuromast hair cell, ameliorated
Day 5 neuromast morphology, ameliorated
Acknowledgments:
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