ZFIN ID: ZDB-FIG-181121-3
Sanchez et al., 2018 - Quinoxaline protects zebrafish lateral line hair cells from cisplatin and aminoglycosides damage. Scientific Reports   8:15119 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Conditions:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Day 5

Fig. 3

Dose protection curve against aminoglycosides. 5dpf larvae were incubated with vehicle (E3, A) or with 50 μM (DF), 150 μM (GI) or 300 μM (JL) of Qx for a total of 8 hours. Gentamicin (GM, 50 μM, B,E,H,K) or neomycin (Neo, 200 µM,C,F,I,L) were added during the last 60 min or 30 min of incubation, respectively. Animals were fixed and stained for otoferlin (red) and GFP (green). (M) Quantification of the number of hair cells per neuromast after the different treatments represented as mean +/− SEM. Note that since no significant differences were found in the number of hair cells per neuromast when animals were incubated with the different Qx concentrations (0–300 µM, A,D,G,J), the control value represents the average of all these treatments. One-way ANOVA, Dunnett post test. *p < 0.05, **p < 0.01, ***p < 0.001. Black asterisks compared versus control. Red asterisks compared versus the corresponding aminoglycoside-only treatment. (N) Scores for neuromast morphology (see Materials and Methods). Scale bar: 7 μm. Data were taken from at least 20 animals and 3 experiments runs.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP s273tTg control Day 5 neuromast hair cell plasma membrane IHC
s273tTg chemical treatment by environment: gentamycin Day 5 neuromast hair cell plasma membrane IHC
s273tTg chemical treatment by environment: neomycin Day 5 neuromast hair cell plasma membrane IHC
s273tTg chemical treatment by environment: quinoxaline Day 5 neuromast hair cell plasma membrane IHC
s273tTg chemical treatment by environment: gentamycin, chemical treatment by environment: quinoxaline Day 5 neuromast hair cell plasma membrane IHC
s273tTg chemical treatment by environment: neomycin, chemical treatment by environment: quinoxaline Day 5 neuromast hair cell plasma membrane IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-otof IHC s273tTg control Day 5 neuromast hair cell
IHC s273tTg chemical treatment by environment: gentamycin Day 5 neuromast hair cell
IHC s273tTg chemical treatment by environment: neomycin Day 5 neuromast hair cell
IHC s273tTg chemical treatment by environment: quinoxaline Day 5 neuromast hair cell
IHC s273tTg chemical treatment by environment: gentamycin, chemical treatment by environment: quinoxaline Day 5 neuromast hair cell
IHC s273tTg chemical treatment by environment: neomycin, chemical treatment by environment: quinoxaline Day 5 neuromast hair cell
Phenotype Details
Fish Conditions Stage Phenotype
s273tTg chemical treatment by environment: gentamycin Day 5 neuromast has fewer parts of type neuromast hair cell, abnormal
Day 5 neuromast malformed, abnormal
s273tTg chemical treatment by environment: neomycin Day 5 neuromast has fewer parts of type neuromast hair cell, abnormal
Day 5 neuromast malformed, abnormal
s273tTg chemical treatment by environment: quinoxaline Day 5 neuromast has normal numbers of parts of type neuromast hair cell, normal
Day 5 neuromast morphology, normal
s273tTg chemical treatment by environment: gentamycin, chemical treatment by environment: quinoxaline Day 5 neuromast has normal numbers of parts of type neuromast hair cell, ameliorated
Day 5 neuromast has number of neuromast hair cell, ameliorated
Day 5 neuromast morphology, ameliorated
s273tTg chemical treatment by environment: neomycin, chemical treatment by environment: quinoxaline Day 5 neuromast has fewer parts of type neuromast hair cell, ameliorated
Day 5 neuromast morphology, ameliorated
Acknowledgments:
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