ZFIN ID: ZDB-FIG-181101-15
Kaufman et al., 2018 - rbpms2 functions in Balbiani body architecture and ovary fate. PLoS Genetics   14:e1007489 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: 4-cell
PHENOTYPE:
Fish:
Observed In:
Stage: 4-cell

Fig. 3

rbpms2 mutant allele stability and localization activity in somatic cells.

(A) RT-PCR to detect rbpms2a and rbpms2b maternal transcripts. Pink asterisks indicate likely nonsense mediated decay of mutant allele transcripts. (B-F) Wild-type GFP-Rbpms2a/b (B, D) and GFP-Rbpms2bsa9329 (F) localize to granules in HEK 293 cells, while GFP-Rbpms2aae30 and GFP-Rbpms2bae32 are not localized. (G-K) Zebrafish blastula cells expressing GFP-Rbpms2 fusions. (G,H) Wild-type GFP-Rbpms2b localizes near the nucleus (H), is apparently associated with the centrosome/spindle in some cells, and (G) is in granules that are not positive for the stress granule marker Tial-1. (H) A subset of GFP-Rbpms2b positive granules are positive for the p-body marker Dcp2 (open arrowheads). (I) GFP-Rbpms2bsa9329 localization to the centrosome/spindle but not granules. (J) GFP-Rbpms2bae32 and (K) GFP-Rbpms2aae30 localization. Insets show magnified views of the highlighted cells. Images are representative slices from Z-stacks of sphere stage embryos viewed from the animal pole.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
rbpms2a AB control 4-cell whole organism RTPCR
rbpms2aae30(AB) control 4-cell whole organism RTPCR
rbpms2bae32(AB) control 4-cell whole organism RTPCR
rbpms2bsa9329(AB) control 4-cell whole organism RTPCR
rbpms2b AB control 4-cell whole organism RTPCR
rbpms2aae30(AB) control 4-cell whole organism RTPCR
rbpms2bae32(AB) control 4-cell whole organism RTPCR
rbpms2bsa9329(AB) control 4-cell whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rbpms2aae30(AB) control 4-cell whole organism rbpms2a expression decreased amount, abnormal
rbpms2bae32(AB) control 4-cell whole organism rbpms2b expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.