Fig. 2

Rbpms2 embryonic phenotype characterization.

(A) rbpms2a and rbpms2b alignment showing extremely similar exon structure, with the pink arrowheads pointing to the Crispr-Cas9 disrupted region of exon 5 in both rbpms2a and rbpms2b, and the blue arrowhead pointing to the disrupted splice site in rbpm2b. (B) Table of germ-line rbpms2 alleles and predicted protein structure, with single and compound mutant phenotypes represented in the two right columns. In the table, n = number of embryos of that genotype, and the number in parentheses represents independent parental crosses from which those embryos were derived. Representative images of the lateral view of d3 embryos, showing head and cardiac structures of non-mutant sibling (C) and rbpms2a^ae30/ae30; rbpms2b^{sa9329/sa9329} double mutant (D). Representative image of lateral view of d5 embryos, showing head and cardiac structures of non-mutant sibling (E) and recovered (F) and edematous (G) rbpms2a^ae30/ae30; rbpms2b^{sa9329/sa9329} double mutants.