Zebrafish rbpms2 mutants are fertile males.
(A-B) Non-mutant siblings of rbpms2 double mutants can differentiate and mature as fertile females with overtly normal oocytes observed when germ cells are marked by ziwi:GFP (A) or with H&E staining (B). (C-D) Non-mutant male siblings of rbpms2 double mutants display normal spermatogenesis with spermatogonia (SG) and spermatozoa (SZ) detectable with ziwi:GFP (C) or with H&E staining (D). (E-F) rbpms2aae30;rbpms2bsa9329 double mutant males have no distinguishable differences in testis morphology or spermatogenic stages from their non-mutant male siblings. (G) Stacked bar chart depicts male to female ratio of adults recovered from each rbpms2a;rbpms2b genotype (blue = male, pink = female).