Community Action Needed: Please respond to the NIH RFI
ZFIN ID: ZDB-FIG-181101-16
Kaufman et al., 2018 - rbpms2 functions in Balbiani body architecture and ovary fate. PLoS Genetics   14:e1007489 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 7

Zebrafish rbpms2 mutants are fertile males.

(A-B) Non-mutant siblings of rbpms2 double mutants can differentiate and mature as fertile females with overtly normal oocytes observed when germ cells are marked by ziwi:GFP (A) or with H&E staining (B). (C-D) Non-mutant male siblings of rbpms2 double mutants display normal spermatogenesis with spermatogonia (SG) and spermatozoa (SZ) detectable with ziwi:GFP (C) or with H&E staining (D). (E-F) rbpms2aae30;rbpms2bsa9329 double mutant males have no distinguishable differences in testis morphology or spermatogenic stages from their non-mutant male siblings. (G) Stacked bar chart depicts male to female ratio of adults recovered from each rbpms2a;rbpms2b genotype (blue = male, pink = female).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP rbpms2aae30/+ ; uc02Tg ; rbpms2bsa9329/+ control Adult germ line cell IHC
Adult oocyte IHC
Adult spermatogonium IHC
rbpms2bsa9329/sa9329 ; uc02Tg ; rbpms2aae30/ae30 control Adult germ line cell IHC
Adult spermatogonium IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rbpms2bae32/ae32 ; rbpms2aae30/ae30 control Adult female organism absent, abnormal
Adult female sex determination decreased occurrence, abnormal
rbpms2bsa9329/sa9329 ; rbpms2aae30/ae30 control Adult female organism absent, abnormal
Adult female sex determination decreased occurrence, abnormal
rbpms2bsa9329/sa9329 ; uc02Tg ; rbpms2aae30/ae30 control Adult female organism absent, abnormal
Adult female sex determination decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.