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ZIRC
ZFIN ID: ZDB-FIG-181023-7
Grassini et al., 2018 - Nppa and Nppb act redundantly during zebrafish cardiac development to confine AVC marker expression and reduce cardiac jelly volume. Development (Cambridge, England)   145(12) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 2

AVC markers are expanded in double mutant embryos and cardiac jelly is thicker. (A) Confocal z-stacks of Tg(myl7:dsRed-nls) embryos (labelling cardiomyocyte nuclei) show no difference in cell number between sibling and double mutant embryos. (A′) Graphical representation of cell number (n=11 siblings; n=14 mutants). (B) Confocal images of representative sibling and double mutant embryos immunostained for Alcama to visualise cell shape. IC, inner curvature (white asterisk); OC, outer curvature (white arrowhead); AVC, atrioventricular canal (white arrow). Individual cardiomyocytes are outlined with broken lines. (B′) Graph showing average cardiomyocyte size of ventricle and atrium in siblings and double mutants (n=11 siblings; n=12 mutants). (C) In situ hybridisation analysis of AVC markers bmp4, tbx2b, has2 and versican are expanded into the atrial chamber of double mutants compared with siblings. (E) Bright-field still images from high-speed movies of hearts at atrial diastole show thickened space (outlined) between the myocardial outer surface and the lumen surface of the heart, indicating thicker cardiac jelly in double mutants. (E′) Quantification of cardiac jelly thickness showing a significant increase in double mutant embryos compared with wild-type siblings when measuring the outer atrial myocardial wall and the inner luminal lining (n=10 siblings; n=11 mutants). ns, not significant; *P<0.05, as determined by t-test. Data are mean±s.d.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
alcama zn-8 WT standard conditions Protruding-mouth cardiac muscle cell plasma membrane IHC
zn-8 nppauq19ks/+ ; nppbuq20ks/+ standard conditions Protruding-mouth cardiac muscle cell plasma membrane IHC
zn-8 nppbuq20ks/uq20ks ; nppauq19ks/uq19ks standard conditions Protruding-mouth cardiac muscle cell plasma membrane IHC
bmp4 WT standard conditions Protruding-mouth atrioventricular canal ISH
nppauq19ks/+ ; nppbuq20ks/+ standard conditions Protruding-mouth atrioventricular canal ISH
nppbuq20ks/uq20ks ; nppauq19ks/uq19ks standard conditions Protruding-mouth atrioventricular canal ISH
Protruding-mouth atrium ISH
Protruding-mouth cardiac muscle cell ISH
DsRed2 f2Tg standard conditions Protruding-mouth cardiac muscle cell nucleus IFL
nppbuq20ks/+ ; f2Tg ; nppauq19ks/+ standard conditions Protruding-mouth cardiac muscle cell nucleus IFL
nppbuq20ks/uq20ks ; nppauq19ks/uq19ks ; f2Tg standard conditions Protruding-mouth cardiac muscle cell nucleus IFL
has2 WT standard conditions Protruding-mouth atrioventricular canal ISH
nppauq19ks/+ ; nppbuq20ks/+ standard conditions Protruding-mouth atrioventricular canal ISH
nppbuq20ks/uq20ks ; nppauq19ks/uq19ks standard conditions Protruding-mouth atrioventricular canal ISH
Protruding-mouth atrium ISH
Protruding-mouth cardiac muscle cell ISH
tbx2b WT standard conditions Protruding-mouth atrioventricular canal ISH
nppauq19ks/+ ; nppbuq20ks/+ standard conditions Protruding-mouth atrioventricular canal ISH
nppbuq20ks/uq20ks ; nppauq19ks/uq19ks standard conditions Protruding-mouth atrioventricular canal ISH
Protruding-mouth atrium ISH
Protruding-mouth cardiac muscle cell ISH
vcana WT standard conditions Protruding-mouth atrioventricular canal ISH
nppauq19ks/+ ; nppbuq20ks/+ standard conditions Protruding-mouth atrioventricular canal ISH
nppbuq20ks/uq20ks ; nppauq19ks/uq19ks standard conditions Protruding-mouth atrioventricular canal ISH
Protruding-mouth atrium ISH
Protruding-mouth cardiac muscle cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nppauq19ks/+ ; nppbuq20ks/+ standard conditions Protruding-mouth cardiac muscle cell normal size, normal
nppbuq20ks/uq20ks ; nppauq19ks/uq19ks standard conditions Protruding-mouth atrioventricular canal development process quality, abnormal
Protruding-mouth atrium vcana expression increased distribution, abnormal
Protruding-mouth atrium tbx2b expression increased distribution, abnormal
Protruding-mouth atrium bmp4 expression increased distribution, abnormal
Protruding-mouth atrium has2 expression increased distribution, abnormal
Protruding-mouth atrium cardiac jelly increased thickness, abnormal
Protruding-mouth atrium cardiac jelly development process quality, abnormal
Protruding-mouth cardiac muscle cell normal size, normal
nppbuq20ks/uq20ks ; nppauq19ks/uq19ks ; f2Tg standard conditions Protruding-mouth heart has normal numbers of parts of type cardiac muscle cell, normal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development