Fig. 1-S1

Knockout of lats1/2 genes leads to an activation of the Tead reporter.

(A) lats1 and lats2 gene mutation by TALEN at the targeted loci. A deletion of 10 bp in the ncv107 allele and 16 bp in the ncv108 allele results in a premature stop codon in exon 3 of lats1 (the resulting mutant Lats1 protein consists of 117 aa) and exon 3 of lats2 (the resulting mutant Lats2 protein consists of 78 aa), respectively. Upper and lower case letters denote the target and spacer regions for the TALEN, respectively. (B) The percentages of double knockout (DKO) embryos obtained by incrossing lats1^wt/ncv107lats2^ncv108 fish at different days post-fertilization (dpf). The total numbers of larvae examined in the experiment are indicated at the top of each column. (C) Fluorescent images (at 28 hpf) of the Tg(eef1a1l1:galdb-hTEAD2ΔN-2A-mCherry);Tg(uas:GFP) embryos injected with control morpholino (control MO, n = 12) and lats1/2 MOs (n = 12) (upper panels), and with lats1^wt/wtlats2^wt/wt (n = 10) or lats1^ncv107lats2^ncv108 alleles (n = 7) (bottom panels). Lateral view, anterior to the left. The fluorescent images are a set of representative images from four independent experiments.