FIGURE

Fig. 3

ID

ZDB-FIG-180821-3

Publication

Bräutigam et al., 2018 - MGST1, a GSH transferase/peroxidase essential for development and hematopoietic stem cell differentiation

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Fig. 3

Loss of functional MGST1 blocks differentiation of hematopoietic stem cells in zebrafish. (A) Histochemical staining of hemoglobin in living zebrafish embryos. (B) Whole-mount in situ hybridization in 48 hpf embryos staining globin transcripts specific for erythrocytes. (C) Whole-mount in situ hybridization against marker genes for different myloid and lymphoid lineages in 96 hpf embryos. (D) RTqPCR quantifying transcripts specific for different myloid and lymphoid lineages in 96 hpf embryos. (E) Quantification and statistics for RTqPCR. Brightfield images were taken on a Leica MZ16 microscope equipped with a Leica DFC300FX camera; images stacks were produced with ImageJ, GIMP was used to adjust the gamma channel.

Expression Data

Genes:	gata1a mpeg1.1 mpx rag1 zfpm1
Fish:	WT WT + MO1-mgst1.1 + MO1-mgst1.2
Knockdown Reagents:	MO1-mgst1.1 MO1-mgst1.2
Anatomical Terms:	macrophage neutrophil nucleate erythrocyte T cell thrombocyte
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-mgst1.1 + MO1-mgst1.2
Knockdown Reagents:	MO1-mgst1.1 MO1-mgst1.2
Observed In:	macrophage neutrophil nucleate erythrocyte T cell thrombocyte
Stage Range:	Long-pec to Day 4

Phenotype Detail

Acknowledgments

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