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Med12 is required for expression of avp, crh and trh and modulates expression of prl and tsh. A-E′ are all dorsal views of WISH in 48 hpf embryos magnified ×100. (A,B,C,D,E) wild-type siblings. (A′,B′,C′,D′,E′) med12 mutants. (A,A′) avp expression in the PO and vH. (B,B′) crh expression in the PO, PT and Lc. (C,C′) trh expression in the PO, vH and Lc. (D,D′) prl expression in the pituitary. (E,E′) tsh expression in the pituitary. (F,G) Expression of prl (F) and tsh (G) were measured in 48 hpf embryos using RT-qPCR. Gene expression values were normalized to an endogenous control, beta actin 2 (actb2), and are expressed as fold changes relative to wild-type sibling controls. **P<0.01, *P<0.05, two-tailed paired Student's t-test. Dot plots show three biological replicates (black) and the mean (red). PO, pre-optic area; PT, posterior tuberculum; Lc, locus coerulus; vH, ventral hypothalamus; Pit, pituitary.
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