Expression of oxt is undetectable in the med12 mutant but is unaffected by treatment with Wnt inhibitors. A-C are all dorsal views of WISH for oxt in 48 hpf embryos magnified ×100 in which arrowheads indicate oxt expression. Embryos treated with IWR and med12 mutants were compared to sibling controls. (A) wild-type embryo. (B) med12 mutant embryo. (C) IWR-treated embryo. (D) oxt expression was measured using RT-qPCR in 48 hpf embryos. Gene expression values were normalized to an endogenous control, beta actin 2 (actb2), and are expressed as fold changes relative to wild-type sibling, or vehicle-treated wild-type sibling controls. A: P<0.01, two-tailed paired Student's t-test. Dot plot shows three biological replicates (black) and the mean (red). (E,E′,F,F′) Representative images of GFP expression was visualized in 24 hpf vehicle and IWR-treated embryos that are heterozygous for the TOP:GFP transgene. (E,E′) Magnified ×100. Arrows indicate area of reduced GFP expression. (F,F′) Representative full embryo views magnified ×50.
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