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ZFIN ID: ZDB-FIG-180503-12
Montague et al., 2017 - Vg1-Nodal heterodimers are the endogenous inducers of mesendoderm. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Sphere to 90%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage Range: Sphere to Prim-5

Fig. 2

Endogenous Nodal signaling requires Vg1.

(A) Mvg1, maternal-zygotic vg1 (MZvg1) and maternal-zygotic oep (MZoep) mutants at 28 hpf. (B) Expression of Nodal target genes gsc, lft1 and ntl at 50% epiboly and sox32 at 90% epiboly in WT, Mvg1 and MZoep embryos. (C) cyc and sqt expression at 4 and 6 hpf in WT and Mvg1 embryos. (D) gsc expression at 50% epiboly in WT and Mvg1 embryos injected with 50 pg of cyc mRNA, 50 pg or 0.2 pg of sqt mRNA. (E) qPCR of lft1 expression at 50% epiboly relative to ef1a in WT and Mvg1 embryos injected with 0.2 pg or 2 pg of sqt mRNA. The mean and standard error of the mean (SEM) was plotted. (F) qPCR of lft1 expression at 50% epiboly relative to ef1a in embryos injected with 20 pg of cyc mRNA in combination with 0.5 pg or 5 pg of vg1 mRNA. The mean and SEM was plotted. (G) Anti-GFP reducing immunoblot of WT and Mvg1 embryos injected with 50 pg of cyc-sfGFP or sqt-sfGFP mRNA. Black arrowhead indicates the position of full-length protein, open arrowhead indicates processed protein. 8 embryos at 50% epiboly were loaded per well. (H) Live imaging of the animal cap of sphere-stage WT and Mvg1 embryos injected with 50 pg of cyc-sfGFP or sqt-sfGFP mRNA. Scale bar, 17 um.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gsc WT standard conditions 50%-epiboly margin ISH
gdf3a164/a164(AB/TL) standard conditions 50%-epiboly Not Detected margin ISH
gdf3a165/a165(AB/TL) standard conditions 50%-epiboly Not Detected margin ISH
tdgf1tz257/tz257 standard conditions 50%-epiboly Not Detected margin ISH
lft1 WT standard conditions 50%-epiboly margin ISH
gdf3a164/+(AB/TL) standard conditions 50%-epiboly whole organism RTPCR
gdf3a164/a164(AB/TL) standard conditions 50%-epiboly Not Detected margin ISH
gdf3a165/+(AB/TL) standard conditions 50%-epiboly whole organism RTPCR
gdf3a165/a165(AB/TL) standard conditions 50%-epiboly Not Detected margin ISH
tdgf1tz257/tz257 standard conditions 50%-epiboly Not Detected margin ISH
ndr1 WT standard conditions Sphere embryonic structure ISH
Shield margin ISH
gdf3a164/+(AB/TL) standard conditions Sphere embryonic structure ISH
Shield margin ISH
gdf3a165/+(AB/TL) standard conditions Sphere embryonic structure ISH
Shield margin ISH
ndr2 WT standard conditions Sphere embryonic structure ISH
Shield margin ISH
gdf3a164/+(AB/TL) standard conditions Sphere embryonic structure ISH
Shield margin ISH
gdf3a165/+(AB/TL) standard conditions Sphere embryonic structure ISH
Shield margin ISH
sox32 WT standard conditions 90%-epiboly hypoblast ISH
gdf3a164/a164(AB/TL) standard conditions 90%-epiboly hypoblast ISH
gdf3a165/a165(AB/TL) standard conditions 90%-epiboly hypoblast ISH
tdgf1tz257/tz257 standard conditions 90%-epiboly hypoblast ISH
tbxta WT standard conditions 50%-epiboly margin ISH
gdf3a164/a164(AB/TL) standard conditions 50%-epiboly margin ISH
gdf3a165/a165(AB/TL) standard conditions 50%-epiboly margin ISH
tdgf1tz257/tz257 standard conditions 50%-epiboly margin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gdf3a164/+(AB/TL) standard conditions Sphere embryonic structure ndr1 expression decreased amount, abnormal
Sphere embryonic structure ndr2 expression decreased amount, abnormal
Shield margin ndr1 expression decreased amount, abnormal
Shield margin ndr2 expression decreased amount, abnormal
50%-epiboly whole organism lft1 expression decreased amount, abnormal
Prim-5 mesendoderm development decreased process quality, abnormal
gdf3a164/a164(AB/TL) standard conditions 50%-epiboly margin lft1 expression absent, abnormal
50%-epiboly margin gsc expression absent, abnormal
50%-epiboly margin tbxta expression decreased amount, abnormal
90%-epiboly hypoblast sox32 expression decreased amount, abnormal
Prim-5 mesendoderm development decreased process quality, abnormal
gdf3a165/+(AB/TL) standard conditions Sphere embryonic structure ndr2 expression decreased amount, abnormal
Sphere embryonic structure ndr1 expression decreased amount, abnormal
Shield margin ndr1 expression decreased amount, abnormal
Shield margin ndr2 expression decreased amount, abnormal
50%-epiboly whole organism lft1 expression decreased amount, abnormal
Prim-5 mesendoderm development decreased process quality, abnormal
gdf3a165/a165(AB/TL) standard conditions 50%-epiboly margin gsc expression absent, abnormal
50%-epiboly margin lft1 expression absent, abnormal
50%-epiboly margin tbxta expression decreased amount, abnormal
90%-epiboly hypoblast sox32 expression decreased amount, abnormal
Prim-5 mesendoderm development decreased process quality, abnormal
tdgf1tz257/tz257 standard conditions 50%-epiboly margin lft1 expression absent, abnormal
50%-epiboly margin gsc expression absent, abnormal
50%-epiboly margin tbxta expression decreased amount, abnormal
90%-epiboly hypoblast sox32 expression decreased amount, abnormal
Prim-5 mesendoderm development decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife