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ZFIN ID: ZDB-FIG-180418-15
Lu et al., 2017 - Twa1/Gid8 is a β-catenin nuclear retention factor in Wnt signaling and colorectal tumorigenesis. Cell Research   27(12):1422-1440 Full text @ Cell Res.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Oblong to Bud
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Oblong to Prim-5

Fig. 7

Zebrafish Twa1 is essential for dorsoventral patterning during embryogenesis. Zebrafish embryos at the one-cell stage injected with the indicated morpholinos targeting Twa1a and Twa1b (Twa1a-MO and Twa1b-MO) or mRNAs were harvested at the different times, and subjected to the following analyses. (A, B) Microscopy showed the different types of embryos at 24 h post-fertilization (hpf). The classes 1-3 represent increasing degrees of phenotypic severity in eye and brain structures. The percentages of embryos with the different classes are shown. (C-F) Whole-mount in situ hybridization of the dorsal (gsc, chordin and otx2) and ventral (gata2 and eve1) markers and the Wnt target gene boz. The percentages of embryos with abnormal expression of the indicated markers are also presented. β-Cat2, β-catenin 2; boz, bozozok; n, number of observed embryos; Twa1a*, zebrafish Twa1a-MO-resistant mRNA; Twa1aCRA*, zebrafish Twa1a-MO-resistant mRNA without CRA domain; Twa1b*, zebrafish Twa1b-MO-resistant mRNA.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
chrd AB control Shield whole organism dorsal region ISH
AB + MO1-gid8b standard conditions Shield whole organism dorsal region ISH
AB + MO3-ctnnb2 standard conditions Shield whole organism dorsal region ISH
dharma AB control Oblong anatomical structure ISH
AB + MO1-gid8b standard conditions Oblong anatomical structure ISH
AB + MO3-ctnnb2 standard conditions Oblong anatomical structure ISH
eve1 AB control Shield whole organism ventral region ISH
AB + MO1-gid8b standard conditions Shield whole organism ventral region ISH
AB + MO3-ctnnb2 standard conditions Shield whole organism ventral region ISH
gata2a AB control Shield whole organism ventral region ISH
AB + MO1-gid8b standard conditions Shield whole organism ventral region ISH
AB + MO3-ctnnb2 standard conditions Shield whole organism ventral region ISH
gsc AB control Shield whole organism dorsal region ISH
AB + MO1-gid8b standard conditions Shield whole organism dorsal region ISH
AB + MO3-ctnnb2 standard conditions Shield whole organism dorsal region ISH
otx2b AB control Bud whole organism dorsal region ISH
AB + MO1-gid8b standard conditions Bud whole organism dorsal region ISH
AB + MO3-ctnnb2 standard conditions Bud whole organism dorsal region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-gid8a standard conditions Prim-5 whole organism ventralized, abnormal
AB + MO1-gid8b standard conditions Oblong anatomical structure dharma expression decreased distribution, abnormal
Shield whole organism dorsal region chrd expression decreased distribution, abnormal
Shield whole organism dorsal region gsc expression decreased distribution, abnormal
Shield whole organism ventral region eve1 expression increased distribution, abnormal
Shield whole organism ventral region gata2a expression increased distribution, abnormal
Bud whole organism dorsal region otx2b expression decreased distribution, abnormal
Prim-5 whole organism ventralized, abnormal
AB + MO3-ctnnb2 standard conditions Oblong anatomical structure dharma expression decreased distribution, abnormal
Shield whole organism dorsal region chrd expression decreased distribution, abnormal
Shield whole organism dorsal region gsc expression decreased distribution, abnormal
Shield whole organism ventral region eve1 expression increased distribution, abnormal
Shield whole organism ventral region gata2a expression increased distribution, abnormal
Bud whole organism dorsal region otx2b expression decreased distribution, abnormal
Prim-5 whole organism ventralized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Research for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Res.