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ZFIN ID: ZDB-FIG-180417-57
Yuan et al., 2017 - Role of WNT10A in failure of tooth development in humans and zebrafish. Molecular genetics & genomic medicine   5:730-741 Full text @ Mol Genet Genomic Med
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 4

Perturbation of wnt10a expression results in altered expression of additional tooth development genes at tooth bud formation stage (2 dpf). (A) Quantitative RTPCR analyses of msx1, dlx2b, eda, and axin2 genes in WT, mismatch‐MO injected (MM), wnt10aMO injected (MO), wnt10a RNA injected (wnt10a), and wnt10a mutant RNA with T328I and G248S mutations (corresponding to T357I and G213S in humans). No differences were observed in the expression of those genes between WT and MM‐injected embryos, whereas knockdown of wnt10a led to significantly decreased expression of dlx2b, eda, and axin2 (P = 0.001). In contrast, overexpression of wnt10a resulted in increased expression of all four genes (P < 0.02), although with significant differences only for msx1 (P = 0.01) and axin2 (P = 0.02). Injection of mutant wnt10a RNAs also perturbed expression of msx1, dlx2b, eda, and axin2, particularly the G248S mutant (0.002 < P < 0.02). Target gene expression was normalized to the expression of beta‐actin used as endogenous control. Fold changes were calculated in relation to controls by the 2−∆∆Ct method and analyzed using Student's t test. P‐values ≤0.05 were considered statistically significant. (B,C) Overexpression of Wnt10a leads to a small/no eyes phenotype in wnt10a RNA‐injected fish (C) in comparison to WT (B) at 2 dpf. Embryos not injected with any RNA (D), injected with 1 pg zebrafish wnt10a RNA (E), 1 pg wnt10a RNA with T382I mutation (F), and 1 pg wnt10a RNA with G248S mutation (G).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
axin2 WT control Long-pec whole organism RTPCR
WT + MO1-wnt10a standard conditions Long-pec whole organism RTPCR
dlx2b WT control Long-pec whole organism RTPCR
WT + MO1-wnt10a standard conditions Long-pec whole organism RTPCR
eda WT control Long-pec whole organism RTPCR
WT + MO1-wnt10a standard conditions Long-pec whole organism RTPCR
msx1a WT control Long-pec whole organism RTPCR
WT + MO1-wnt10a standard conditions Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-wnt10a standard conditions Long-pec whole organism axin2 expression decreased amount, abnormal
Long-pec whole organism dlx2b expression decreased amount, abnormal
Long-pec whole organism eda expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Molecular genetics & genomic medicine for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Mol Genet Genomic Med