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ZFIN ID: ZDB-FIG-180411-11
Veil et al., 2017 - Maternal Nanog is critical for the zebrafish embryo architecture and for cell viability during gastrulation. Development (Cambridge, England)   145(1) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1k-cell to 75%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage Range: 1k-cell to 75%-epiboly

Fig. 7 Nanog is required for the expression of the ventral genes bmp2b, vox and vent and for the proper expression levels of the neural gene her3. (A-O) In situ hybridization for dorsal- and ventral-specifying genes was performed in parallel using wild type (WT), MZnanog and MZspg at the shield stage. The dorsal markers gsc (A,F,K) and chrd (B,G,L) are slightly reduced in MZnanog embryos. vent expression (C,H,M) is not detectable in MZnanog but is visible although reduced in MZspg. vox (D,I,N) and bmp2b (E,J,O) expression is reduced in the mesoderm and missing from the ectoderm of MZnanog (arrowheads), whereas in MZspg expression in both tissues is reduced, but detectable. (P) In situ hybridization at 4.7 hpf for her3 in WT and MZnanog (animal view). At this stage, her3 in the WT is expressed in presumptive neural ectoderm (Hans et al., 2004). Because of the upregulation in the MZnanog mutant, the staining reaction was stopped before this staining was visible. (Q) her3 time curve generated by quantitative real-time PCR on WT, MZnanog and MZspg. Embryos were collected at 1 h intervals from 3 hpf to 8 hpf. All expression values were normalized to her3 maximal expression in WT (7 hpf). Error bars represent s.d.; n=3. (R,S) Overexpression of Nanog in Tg(her3enh1-GFP) embryos changes her3 levels. We injected nanog or control mRNA in Tg(her3enh1-GFP) transgenic embryos at the 1-cell stage and performed quantitative RT-PCR on shield stage-embryos measuring changes of her3 and GFP RNA levels. Overexpression of Nanog did not change GFP levels (R) but repressed endogenous her3 (**P<0.001, ***P<1*E−05, Student's t-test) (S). Error bars represent s.d., n=3. Scale bars: 700 µm (A-O); 100 µm (P).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bmp2b WT standard conditions Shield epiblast ISH
nanogm1435/m1435 standard conditions Shield Not Detected epiblast ISH
pou5f3m793/m793 standard conditions Shield epiblast ISH
chrd WT standard conditions Shield whole organism dorsal region ISH
nanogm1435/m1435 standard conditions Shield whole organism dorsal region ISH
pou5f3m793/m793 standard conditions Shield whole organism dorsal region ISH
gsc WT standard conditions Shield whole organism dorsal region ISH
nanogm1435/m1435 standard conditions Shield whole organism dorsal region ISH
pou5f3m793/m793 standard conditions Shield whole organism dorsal region ISH
her3 WT standard conditions 30%-epiboly DEL ISH
nanogm1435/m1435 standard conditions 1k-cell to 75%-epiboly whole organism ISH
30%-epiboly DEL ISH
vent WT standard conditions Shield whole organism ventral region ISH
nanogm1435/m1435 standard conditions Shield whole organism ventral region ISH
pou5f3m793/m793 standard conditions Shield whole organism ventral region ISH
vox WT standard conditions Shield epiblast ISH
Shield whole organism ventral region ISH
nanogm1435/m1435 standard conditions Shield Not Detected epiblast ISH
Shield whole organism ventral region ISH
pou5f3m793/m793 standard conditions Shield epiblast ISH
Shield whole organism ventral region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nanogm1435/m1435 standard conditions 1k-cell - 75%-epiboly whole organism her3 expression increased amount, abnormal
30%-epiboly DEL her3 expression increased amount, abnormal
Shield epiblast bmp2b expression absent, abnormal
Shield epiblast vox expression absent, abnormal
Shield whole organism ventral region vent expression decreased amount, abnormal
Shield whole organism ventral region vox expression decreased amount, abnormal
pou5f3m793/m793 standard conditions Shield epiblast bmp2b expression decreased amount, abnormal
Shield epiblast vox expression decreased amount, abnormal
Shield whole organism ventral region vox expression decreased amount, abnormal
Shield whole organism ventral region vent expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development