Downregulation or mutations of Itga8 or Pcdh15a inhibits ciliogenesis. (A?J) SR-SIM of 3?dpf (A?G) and 5?dpf (H?J) larvae immunostained for acetylated tubulin (ac tubulin, red) and counterstained with phalloidin (green). Control morpholino-injected animals (A), itga8 (Itga8 MO, B) or pcdh15a (15a MO, C) morphants, morphants co-injected with itga8 (8 MO+cRNA; D) or pcdh15a (15a MO+cRNA; E) cRNAs, Itga8 morphants co-injected with CA rhoab (+Rhoab; F) or rhoad (+Rhoad; G) cRNAs, WT (H), and orbiter (orb) strong (I) and orbiter weak mutants (J) are shown. Scale bars: 4?µm (A?G), 3.5?µm (H?J). (K,L) Quantification analysis of the kinociliary length. For each independent experiment, the average kinociliary length per neuromast was calculated and expressed as meanħs.e.m. (M) Scatter plot of individual kinociliary lengths for WT and orbiter mutants. (N) Frequency distribution analyses of kinociliary length in WT and orbiter mutants. (O) Cartoon of a neuromast (top view) showing the stained structures: hair bundle in green and kinocilia in red. **P<0.01; ***P<0.001; ns, not significant (one-way ANOVA followed by Dunnett's multiple comparisons test or two-tailed Student's t-test). At least five independent experiments were performed.
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