ZFIN ID: ZDB-FIG-180222-21
Akerberg et al., 2017 - Histone demethylases Kdm6ba and Kdm6bb redundantly promote cardiomyocyte proliferation during zebrafish heart ventricle maturation. Developmental Biology   426(1):84-96 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Days 7-13

Fig. 4 Kdm6ba and kdm6bb redundantly promote cardiac trabeculation. (A, C) Fluorescent imaged whole mount 72 hpf embryos showing the aortic arch arteries of control and kdm6b-deficient Tg(kdrl:EGFP) animals. (B, D) Confocal heart images of whole mount 72 hpf Tg(myl7:DsRed-nuc)larvae antibody stained for DsRed (red, cardiomyocyte nuclei) and cardiac troponin (CT3, heart muscle). Control and combined kdm6ba/kdm6bb-deficient larvae are shown. (E) Bar graphs comparing the absolute number of atrial and ventricular cardiomyocytes between 72 hpf control (kdm6ba+/-; kdm6bb+/- and kdm6ba+/+; kdm6bb+/-) and kdm6ba-/-; kdm6bb-/- larvae. Error bars represent one standard deviation (n=6 control and 7 kdm6ba/bb-deficient fish from two clutches). (F, G) DIC microscopy images showing the OFT and ventricle of control and kdm6b-deficient embryos at 5 dpf. Dashed yellow lines outline the OFT valves. (H, I) Whole mount confocal imaged hearts of immunostained 5 dpf control and kdm6ba-/-; kdm6bb-/- larvae. These Tg(myl7:DsRed-nuc) fish are stained with anti-DsRed (red, cardiomyocyte nuclei) and anti-cardiac troponin (CT3, green) antibodies. (J-M) H&E stained sagittal sections through the heart of 5 and 7 dpf control and kdm6ba-/-; kdm6bb-/- larvae. Solid lines outline the trabeculae and dashed yellow lines indicate the AVC valves. (N, O) Scatterplot graphs showing the normalized outer curvature ventricular sectional area and trabeculation extent (“index”) of 5 and 7 dpf control compared to clutch mate kdm6ba-/-; kdm6bb-/- larvae. Each data point represents one animal. P-values are from Student's two-tailed t-tests. (P) Bar graph of qRT-PCR data comparing kdm6ba and kdm6bb to vmhcl transcript levels using three pools of isolated 3 dpf zebrafish embryonic hearts (normalized to vmhcl levels). Error bars are one standard deviation.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
kdm6ba AB standard conditions Protruding-mouth heart RTPCR
kdm6bb AB standard conditions Protruding-mouth heart RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kdm6bab1308/b1308; kdm6bbb1307/b1307 standard conditions Day 5 bulbus arteriosus morphology, normal
Day 5 cardiac ventricle decreased size, abnormal
Day 5 cardiac ventricle shape, abnormal
Day 5 heart trabecula formation decreased process quality, abnormal
Days 7-13 cardiac ventricle decreased size, abnormal
Days 7-13 cardiac ventricle shape, abnormal
Days 7-13 heart trabecula formation decreased process quality, abnormal
kdm6bab1308/b1308; kdm6bbb1307/b1307; hsc4Tg standard conditions Day 5 cardiac ventricle decreased size, abnormal
Day 5 cardiac ventricle shape, abnormal
kdm6bab1308/b1308; kdm6bbb1307/b1307; s843Tg standard conditions Protruding-mouth atrium cardiac muscle cell amount, normal
Protruding-mouth cardiac ventricle cardiac muscle cell amount, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 426(1), Akerberg, A.A., Henner, A., Stewart, S., Stankunas, K., Histone demethylases Kdm6ba and Kdm6bb redundantly promote cardiomyocyte proliferation during zebrafish heart ventricle maturation, 84-96, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.