ZFIN ID: ZDB-FIG-180222-19
Akerberg et al., 2017 - Histone demethylases Kdm6ba and Kdm6bb redundantly promote cardiomyocyte proliferation during zebrafish heart ventricle maturation. Developmental Biology   426(1):84-96 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 2 Generation and characterization of kdm6b loss of function alleles. (A) Schematic showing the deleted sequence in the kdm6bb mutant allele recovered from CRISPR/Cas9 targeted mutagenesis (magenta bases). (B-E) Whole mount differential interference contrast (DIC) microscopy images of 24 (B and D) and 48 hpf (C and E) embryos. (F) Bar graph showing relative kdm6bb transcript levels between wildtype and kdm6bbMZ embryos at 30 hpf determined by qRT-PCR on cDNA from three separate pools of ten embryos each. (G, H) In situ hybridization for kdm6bb on control and kdm6bbMZ 24 hpf zebrafish embryos. (I) Schematic showing the CRISPR/Cas9-generated kdm6ba mutant allele. The deleted base is shown in magenta. (J-M) DIC images of control and kdm6baMZ embryos at 24 hpf (J and L) and 48 hpf (K and M). (N) Bar graphs comparing qRT-PCR-determined normalized expression levels of each H3K27me3 demethylase-encoding transcripts between 30 hpf wildtype and kdm6bbMZ embryos (three independent pools of ten embryos each). Error bars in the bar graphs (F, N) show one standard deviation. P-values indicate a significant difference determined by Student's two-tailed t-tests after applying a Bonferroni correction for multiple comparisons.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
kdm6a WT standard conditions Prim-15 whole organism RTPCR
kdm6bab1308/b1308 standard conditions Prim-15 whole organism RTPCR
kdm6bbb1307/b1307 standard conditions Prim-15 whole organism RTPCR
kdm6al WT standard conditions Prim-15 whole organism RTPCR
kdm6bab1308/b1308 standard conditions Prim-15 whole organism RTPCR
kdm6bbb1307/b1307 standard conditions Prim-15 whole organism RTPCR
kdm6ba WT standard conditions Prim-15 whole organism RTPCR
kdm6bab1308/b1308 standard conditions Prim-15 whole organism RTPCR
kdm6bbb1307/b1307 standard conditions Prim-15 whole organism RTPCR
kdm6bb WT standard conditions Prim-15 whole organism RTPCR
kdm6bab1308/b1308 standard conditions Prim-15 whole organism RTPCR
kdm6bbb1307/+ standard conditions Prim-5 anatomical structure ISH
kdm6bbb1307/b1307 standard conditions Prim-5 anatomical structure ISH
Prim-15 whole organism ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kdm6bab1308/b1308 standard conditions Prim-5 whole organism morphology, normal
Prim-15 whole organism kdm6ba expression decreased amount, abnormal
Long-pec whole organism morphology, normal
kdm6bbb1307/b1307 standard conditions Prim-5 anatomical structure kdm6bb expression decreased amount, abnormal
kdm6bbb1307/b1307 standard conditions Prim-5 whole organism morphology, normal
kdm6bbb1307/b1307 standard conditions Prim-15 whole organism kdm6bb expression decreased amount, abnormal
kdm6bbb1307/b1307 standard conditions Long-pec whole organism morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 426(1), Akerberg, A.A., Henner, A., Stewart, S., Stankunas, K., Histone demethylases Kdm6ba and Kdm6bb redundantly promote cardiomyocyte proliferation during zebrafish heart ventricle maturation, 84-96, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.