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ZFIN ID: ZDB-FIG-180208-4
Donat et al., 2018 - Heg1 and Ccm1/2 proteins control endocardial mechanosensitivity during zebrafish valvulogenesis. eLIFE   7:e28939 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 1

Mechanosensitive expression of heg1 mRNA and stabilization of Krit1 protein levels by heg1.

(A) RT-qPCR quantifications of heg1 and krit1 mRNA levels at 54 hpf in morphant embryos lacking blood-flow [troponin T type 2a (tnnt2a)], or Krüppel like factors 2a and 2b (klf2a/b) compared with wild-type expression levels. (B) RT-qPCR quantification showing that heat-shock-induced overexpression of klf2a causes significant upregulation of heg1 but not of krit1 expression at 54 hpf. (C–E) Morpholino-mediated knockdown of Heg1 significantly reduces endocardial EGFP-Krit1 protein levels of 30 hpf Tg(fli:GAL4FF)ubs3; Tg(UAS:EGFP-Krit1)pbb21 embryos. Scale bars are 50 µm. Mean values ± SEM of three (tnnt2a MO, hs:klf2a, heg1 MO) or four (klf2a/b MO) individual experiments are shown. Ratio paired (A, B) or unpaired (E) t-test was used to compare each condition with controls (ns: not significant, *p<0.05, ***p<0.001).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP pbb21Tg ; ubs3Tg standard conditions Prim-15 endocardium IFL
pbb21Tg ; ubs3Tg + MO1-heg1 standard conditions Prim-15 endocardium IFL
heg1 WT + MO1-tnnt2a standard conditions Long-pec whole organism RTPCR
WT + MO2-klf2a + MO2-klf2b standard conditions Long-pec whole organism RTPCR
pbb22Tg heat shock Long-pec whole organism RTPCR
krit1 WT + MO1-tnnt2a standard conditions Long-pec whole organism RTPCR
WT + MO2-klf2a + MO2-klf2b standard conditions Long-pec whole organism RTPCR
pbb22Tg heat shock Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tnnt2a standard conditions Long-pec blood circulation arrested, abnormal
Long-pec whole organism heg1 expression decreased amount, abnormal
WT + MO2-klf2a + MO2-klf2b standard conditions Long-pec blood circulation arrested, abnormal
Long-pec whole organism heg1 expression decreased amount, abnormal
pbb21Tg ; ubs3Tg + MO1-heg1 standard conditions Prim-15 endocardium EGFP expression decreased amount, abnormal
pbb22Tg heat shock Long-pec whole organism heg1 expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife