|
Fig. S1
Dual reporter system used for insertional mutagenesis, the golden fish project. (A) Body color change in MCH-expressing transgenic zebrafish (TG, a cmv:mch tg line), compared to wild-type adult fish (WT). (B) Expression of dual reporter, GFP-MCH fusion protein, in ef1a:gfp-mch-injected zebrafish embryos. GFP fluorescence and melanosome aggregation are detectable in injected embryos, inj (+), compared to un-injected control embryo, inj (-). (C) Structure of a gene trap vector used in insertional mutagenesis. IR/DR; inverted repeat/direct repeat element; ef1a, elongation factor 1 alpha (ef1a) gene promoter; sp, signal peptide; GFP; green fluorescent protein; MCH, melanin-concentrating hormone; SD, splice donor. (D) Representative mutants showing GFP expression in various tissues (ExTSD1-7; images not to scale). (E) Mapping of the insertion site (arrow) in the intron 3 of a samdori gene on chromosome 4.