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ZFIN ID: ZDB-FIG-180122-17
Han et al., 2017 - Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-β2 nuclear import system. PLoS Biology   15:e2002063 Full text @ PLoS Biol.
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Bud to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Bud to Prim-5

Fig. 5

Karyopherinβ2 (Kapβ2) regulates Hedgehog (Hh) signaling in zebrafish embryos.

(A–C′) Zebrafish embryos injected with the indicated morpholinos (MOs) were immunostained at 24 hours post-fixation (hpf) with Engrailed (Eng) antibody (red) and DAPI (blue) to visualize nuclei. (D–F) Zebrafish embryos injected with the indicated MOs were immunostained at 24 hpf with F59 antibody (red) to visualize slow muscle fibers. (G–I) Zebrafish embryos injected with the indicated MOs were analyzed for Ptch2 expression at 10 hpf by in situ hybridization. (J) Relative mRNA levels of the indicated genes from 24 hpf zebrafish embryos injected with the indicated MOs were measured by quantitative reverse transcription PCR (RT-qPCR). Data are means ± SD from 3 independent experiments. *P < 0.05, ***P < 0.001, NS: not significant. The underlying data for this figure can be found in S1 Data.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
axin2 AB control Prim-5 whole organism RTPCR
AB + MO1-tnpo1 + MO2-tnpo1 standard conditions Prim-5 whole organism RTPCR
foxa AB control Prim-5 whole organism RTPCR
AB + MO1-tnpo1 + MO2-tnpo1 standard conditions Prim-5 whole organism RTPCR
gli1 AB control Prim-5 whole organism RTPCR
AB + MO1-tnpo1 + MO2-tnpo1 standard conditions Prim-5 whole organism RTPCR
hhip AB control Prim-5 whole organism RTPCR
AB + MO1-tnpo1 + MO2-tnpo1 standard conditions Prim-5 whole organism RTPCR
nkx2.2b AB control Prim-5 whole organism RTPCR
AB + MO1-tnpo1 + MO2-tnpo1 standard conditions Prim-5 whole organism RTPCR
ptch2 AB control Bud adaxial cell ISH
Bud whole organism ISH
Prim-5 whole organism RTPCR
AB + MO1-tnpo1 + MO2-tnpo1 standard conditions Bud adaxial cell ISH
Prim-5 whole organism RTPCR
AB + MO8-smo standard conditions Bud adaxial cell ISH
tnpo1 AB control Prim-5 whole organism RTPCR
AB + MO1-tnpo1 + MO2-tnpo1 standard conditions Prim-5 whole organism RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-eng IHC AB control Prim-5 somite
IHC AB + MO1-tnpo1 + MO2-tnpo1 standard conditions Prim-5 somite
IHC AB + MO8-smo standard conditions Prim-5 somite
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-tnpo1 + MO2-tnpo1 standard conditions Bud adaxial cell ptch2 expression decreased amount, abnormal
Prim-5 slow muscle cell morphology, abnormal
Prim-5 somite ab1-eng labeling decreased amount, abnormal
Prim-5 somite U-shaped, abnormal
Prim-5 whole organism nkx2.2b expression decreased amount, abnormal
Prim-5 whole organism hhip expression decreased amount, abnormal
Prim-5 whole organism foxa expression decreased amount, abnormal
Prim-5 whole organism gli1 expression decreased amount, abnormal
Prim-5 whole organism tnpo1 expression decreased amount, abnormal
Prim-5 whole organism ptch2 expression decreased amount, abnormal
AB + MO8-smo standard conditions Bud adaxial cell ptch2 expression decreased amount, abnormal
Prim-5 slow muscle cell morphology, abnormal
Prim-5 somite ab1-eng labeling decreased amount, abnormal
Prim-5 somite U-shaped, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.