FIGURE

Fig. 1

ID

ZDB-FIG-171227-3

Publication

Shao et al., 2017 - Vegetally localised Vrtn functions as a novel repressor to modulate bmp2b transcription during dorsoventral patterning in zebrafish.

Other Figures

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Fig. 1

VAb@2cell inhibits BMP signalling. (A,B) Decreased P-Smad1/5/8 immunostaining (green), merged with DAPI (blue), in VAb@2cell embryos at 60% epiboly. (C,D) Analysis by western blotting of P-Smad1/5/8 levels in VAb@2cell and VAb@1cell embryos at 70% epiboly. P-Smad1/5/8 levels are normalised to β-actin, and BMP activity in intact embryos is set as 1. Data are mean±s.d. from three independent experiments. (E-G) Analysis by in situ hybridisation of indicated genes. Animal pole views with dorsal at the top. (H) Procedure of cell transplantation to visualise cell non-autonomous inhibition of BMP signalling in VAb@2cell embryos using P-Smad1/5/8 immunofluorescence (IF) staining. (I) RLDx-labelled donor cells transplanted at different locations of the margin in unlabelled intact embryos at 70% epiboly. (J-O) Similar presence of P-Smad1/5/8 immunostaining in transplanted cells derived from intact (J-L) or VAb@2cell (M-O) embryos. Scale bars: 250 µm in A,B,E-G; 20 µm in J-O.

Expression Data

Genes:	eve1 gata2a gsc tbxta
Antibody:	Ab1-smad
Fish:	WT
Condition:	ablation: yolk
Anatomical Terms:	anatomical structure whole organism
Stage:	Shield

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	ablation: yolk
Observed In:	anatomical structure BMP signaling pathway whole organism
Stage Range:	50%-epiboly to Shield

Phenotype Detail

Acknowledgments

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