ZFIN ID: ZDB-FIG-171227-3
Shao et al., 2017 - Vegetally localised Vrtn functions as a novel repressor to modulate bmp2b transcription during dorsoventral patterning in zebrafish.. Development (Cambridge, England)   144:3361-3374 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage Range: Germ-ring to Shield
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Shield

Fig. 1

VAb@2cell inhibits BMP signalling. (A,B) Decreased P-Smad1/5/8 immunostaining (green), merged with DAPI (blue), in VAb@2cell embryos at 60% epiboly. (C,D) Analysis by western blotting of P-Smad1/5/8 levels in VAb@2cell and VAb@1cell embryos at 70% epiboly. P-Smad1/5/8 levels are normalised to β-actin, and BMP activity in intact embryos is set as 1. Data are mean±s.d. from three independent experiments. (E-G) Analysis by in situ hybridisation of indicated genes. Animal pole views with dorsal at the top. (H) Procedure of cell transplantation to visualise cell non-autonomous inhibition of BMP signalling in VAb@2cell embryos using P-Smad1/5/8 immunofluorescence (IF) staining. (I) RLDx-labelled donor cells transplanted at different locations of the margin in unlabelled intact embryos at 70% epiboly. (J-O) Similar presence of P-Smad1/5/8 immunostaining in transplanted cells derived from intact (J-L) or VAb@2cell (M-O) embryos. Scale bars: 250 µm in A,B,E-G; 20 µm in J-O.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
eve1 WT standard conditions Shield anatomical structure ISH
WT ablation: yolk Shield anatomical structure ISH
gata2a WT standard conditions Germ-ring to Shield anatomical structure ISH
WT ablation: yolk Germ-ring to Shield anatomical structure ISH
gsc WT standard conditions Germ-ring to Shield anatomical structure ISH
WT ablation: yolk Germ-ring to Shield anatomical structure ISH
tbxta WT standard conditions Shield anatomical structure ISH
WT ablation: yolk Shield anatomical structure ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-smad IHC WT standard conditions 50%-epiboly - Shield anatomical structure
IHC WT ablation: yolk 50%-epiboly - Shield anatomical structure
WB 50%-epiboly - Shield whole organism
Phenotype Details
Fish Conditions Stage Phenotype
WT ablation: yolk Shield anatomical structure eve1 expression decreased amount, abnormal
50%-epiboly - Shield anatomical structure ab1-smad labeling decreased amount, abnormal
Germ-ring - Shield anatomical structure gata2a expression decreased amount, abnormal
Germ-ring - Shield anatomical structure gsc expression decreased amount, abnormal
Germ-ring - Shield anatomical structure gsc expression spatial pattern, abnormal
Shield BMP signaling pathway decreased occurrence, abnormal
50%-epiboly - Shield BMP signaling pathway decreased occurrence, abnormal
Germ-ring - Shield BMP signaling pathway decreased occurrence, abnormal
50%-epiboly - Shield whole organism ab1-smad labeling decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development