Fig. 6

AT7519 and CDK9 morpholino knockdown has no effect on macrophage accumulation post-injury.

Tg(MPEG1:mCherry) zebrafish underwent tailfin transection and were treated with DMSO (control) or AT7519 at 4?hpi. (a) Example images of DMSO [i] and AT7519 [ii] ? treated animals at 24?hpi are shown with cumulative data in (b). ?22 fish per group over 3 independent experiments. Newly laid Tg(MPEG1:mCherry) zebrafish eggs were also injected with a CDK9 splice-blocking morpholino or mismatched control sequence, then raised to 3?dpf. (c) The embryo tailfin was transected and the fish imaged at various time points with example images shown at 24?hpi for morpholino [i] and control [ii] fish and cumulative data shown in (d). n???20 fish from 3 independent experiments. Data shown as ±S.E.M. *p?<?0.05, n/s: not significant, assessed by two-way ANOVA followed by post-hoc Newman Keuls test.