Fig. 4

CDK9 knockdown, but not CDK7 knockdown, reduces neutrophilic inflammation.

Tg(mpx:EGFP)ⁱ¹¹⁴ zebrafish eggs were injected with a CDK9-targeting morpholino or mismatched control sequence and raised to 3 dpf. Embryos with good morpholino uptake were screened, and underwent tailfin transection prior to serial imaging of the wound. (a) Representative images of the tailfin from 24 hpi in mismatch [i] or morpholino sequence-injected fish [ii] are shown, with cumulative data (b [i]). n ≥ 38 fish from 4 independent experiments. (b) The total neutrophils in the whole embryo at 3 dpf were counted [ii] and the percentage of apoptotic neutrophils at 8 hpi was calculated by quantifying TSA⁺/TUNEL⁺ double positive cells as a percentage of total TSA⁺ cells [iii]. Tg(mpx:EGFP)ⁱ¹¹⁴ zebrafish eggs were similarly injected with a CDK7-targeting morpholino or mismatched control sequence. (c) The tailfin was transected at 3 dpf and imaged at 0, 4 and 24 hpi with cumulative data shown. ≥16 fish per group in 3 independent experiments. *p < 0.05, n/s: not significant, analysed two-way ANOVA followed by post-hoc Newman Keuls test.