Fig. 4

CDK9 knockdown, but not CDK7 knockdown, reduces neutrophilic inflammation.

Tg(mpx:EGFP)ⁱ¹¹⁴ zebrafish eggs were injected with a CDK9-targeting morpholino or mismatched control sequence and raised to 3?dpf. Embryos with good morpholino uptake were screened, and underwent tailfin transection prior to serial imaging of the wound. (a) Representative images of the tailfin from 24?hpi in mismatch [i] or morpholino sequence-injected fish [ii] are shown, with cumulative data (b [i]). n???38 fish from 4 independent experiments. (b) The total neutrophils in the whole embryo at 3?dpf were counted [ii] and the percentage of apoptotic neutrophils at 8?hpi was calculated by quantifying TSA⁺/TUNEL⁺ double positive cells as a percentage of total TSA⁺ cells [iii]. Tg(mpx:EGFP)ⁱ¹¹⁴ zebrafish eggs were similarly injected with a CDK7-targeting morpholino or mismatched control sequence. (c) The tailfin was transected at 3?dpf and imaged at 0, 4 and 24?hpi with cumulative data shown. ?16 fish per group in 3 independent experiments. *p?<?0.05, n/s: not significant, analysed two-way ANOVA followed by post-hoc Newman Keuls test.