FIGURE

Fig. 5

ID

ZDB-FIG-171128-12

Publication

Hoodless et al., 2016 - Genetic and pharmacological inhibition of CDK9 drives neutrophil apoptosis to resolve inflammation in zebrafish in vivo

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

CRISPR/cas9 knockout of CDK9 reduces neutrophilic inflammation.

Tg(mpx:EGFP)ⁱ¹¹⁴ zebrafish eggs were micro-injected with cas9 mRNA and a guide mRNA to target CDK9. (a) These animals were raised and in-crossed, then PCR and restriction digests with the restriction enzyme Hpy188i were carried out to assess if embryos were homozygote (Hom), wild type (WT) or heterozygous (Het). The black dividing line is used to show this is two example images cropped from different areas of the same gel. (b) Total embryonic neutrophils in fish from each group of embryos were counted. (c,d) The tailfin of WT, Hom and Het embryos were transected and the neutrophils at the site of wounding were imaged at various time points and quantified. ≥47 fish per group from 3 independent experiments. Data shown as ±S.E.M. *p < 0.05, n/s: not significant, assessed by two-way ANOVA followed by post-hoc Newman Keuls test.

Expression Data

Gene:	GFP
Fish:	i114Tg cdk9^ed9/+; i114Tg cdk9^ed9/ed9; i114Tg
Condition:	transection: caudal fin
Anatomical Term:	neutrophil
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cdk9^ed9/+; i114Tg cdk9^ed9/ed9; i114Tg
Condition:	transection: caudal fin
Observed In:	regenerating fin neutrophil whole organism neutrophil
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.