FIGURE

Fig. 3

ID

ZDB-FIG-171128-10

Publication

Hoodless et al., 2016 - Genetic and pharmacological inhibition of CDK9 drives neutrophil apoptosis to resolve inflammation in zebrafish in vivo

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Fig. 3

CDK inhibition induces neutrophil apoptosis at the site of tailfin injury in vivo.

(a) TSA/TUNEL staining was carried out on Tg(mpx:EGFP)ⁱ¹¹⁴ zebrafish following tailfin transection and either DMSO [i] or AT7519 [ii] treatment, labelling neutrophils in green, and apoptotic cells in red, with representative images shown. (b) Apoptotic neutrophils (double positive red and green) are shown by the arrows. The absolute number of double positive cells were quantified at 12 hpi. ≥16 embryos per group in 3 independent experiments. *p < 0.05 12 h DMSO vs 12 h AT7519, unpaired t test. n/s: not significant. Data expressed as ±S.E.M.

Expression Data

Gene:	GFP
Fish:	i114Tg
Condition:	chemical treatment by injection: 4-(2,6-dichlorobenzamido)-N-(piperidin-4-yl)-pyrazole-3-carboxamide, transection: caudal fin
Anatomical Term:	neutrophil
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	i114Tg
Condition:	chemical treatment by injection: 4-(2,6-dichlorobenzamido)-N-(piperidin-4-yl)-pyrazole-3-carboxamide, transection: caudal fin
Observed In:	neutrophil apoptotic process
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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