ZFIN Image: Hoodless et al., 2016, Fig. 5

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Figure Caption

Fig. 5

CRISPR/cas9 knockout of CDK9 reduces neutrophilic inflammation.

Tg(mpx:EGFP)ⁱ¹¹⁴ zebrafish eggs were micro-injected with cas9 mRNA and a guide mRNA to target CDK9. (a) These animals were raised and in-crossed, then PCR and restriction digests with the restriction enzyme Hpy188i were carried out to assess if embryos were homozygote (Hom), wild type (WT) or heterozygous (Het). The black dividing line is used to show this is two example images cropped from different areas of the same gel. (b) Total embryonic neutrophils in fish from each group of embryos were counted. (c,d) The tailfin of WT, Hom and Het embryos were transected and the neutrophils at the site of wounding were imaged at various time points and quantified. ≥47 fish per group from 3 independent experiments. Data shown as ±S.E.M. *p < 0.05, n/s: not significant, assessed by two-way ANOVA followed by post-hoc Newman Keuls test.

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Acknowledgments

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