FIGURE

Fig. 2

ID

ZDB-FIG-171117-22

Publication

Halluin et al., 2016 - Habenular Neurogenesis in Zebrafish Is Regulated by a Hedgehog, Pax6 Proneural Gene Cascade

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Fig. 2

Pax6a but not Pax6b are required for habenular neurogenesis.

Whole-mount in situ hybridization against cxcr4b (A-D) or brn3a (E-H) showing the epithalamus of wild type (A,E), pax6b^sa86 mutant embryos (B,F), pax6a morpholino injected embryos (C,G) or pax6b^sa8;pax6a morphant embryos (D,H) at 36 (A-D) or 48 (E-H) hpf. Whereas the expression of cxcr4b and brn3a appears unaffected in pax6b^sa86 mutant embryos (B, n = 6/6 and F, n = 5/5) compared to the expression in wild type controls (A and E, n = 5/5), expression of both genes is abrogated after injection of morpholinos against pax6a in either wild type (C, n = 4/6 and G, n = 6/8) or pax6b^sa8 embryos (D and H, n = 4/4). Embryos are viewed dorsally with anterior up.

Expression Data

Genes:	cxcr4b pou4f1
Fish:	WT pax6b^sa86/sa86 WT + MO5-pax6a pax6b^sa86/sa86 + MO5-pax6a
Knockdown Reagent:	MO5-pax6a
Anatomical Terms:	epithalamus habenula
Stage Range:	Prim-25 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO5-pax6a pax6b^sa86/sa86 + MO5-pax6a
Knockdown Reagent:	MO5-pax6a
Observed In:	epithalamus habenula
Stage Range:	Prim-25 to Long-pec

Phenotype Detail

Acknowledgments

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