Fig. S3
Hh signalling is required for the expression of pax6a, neurog1 and neurod4 in the epithalamus. Whole-mount in situ hybridization against pax6a (A,B), neurog1 (C,D) and neurod4 (E,F) showing the head of control treated embryos (A, n = 10; C, n = 12; E, n = 8) or those treated from 16 hpf with cyclopamine (B, n = 10; D, n = 13; F, n = 9) in a lateral view of embryonic heads; all embryos are at 36 hpf and shown with anterior to the left. The expression of pax6a, neurog1 and neurod4 appears perturbed in the most dorsal part of the diencephalon subdivision (black brackets), while their expression does not appear significantly changed more ventrally or in other brain regions, such as the telencephalon (show as a *). |