FIGURE

Fig. 6

ID
ZDB-FIG-171108-22
Publication
Zhou et al., 2016 - Cardiac sodium channel regulator MOG1 regulates cardiac morphogenesis and rhythm
Other Figures
All Figure Page
Back to All Figure Page
Fig. 6

Knockdown of mog1 causes a decrease of expression levels of cardiac transcriptional factors in the anterior lateral mesoderm by whole-mount in situ hybridization.

(A–D) Knockdown of mog1 expression reduces intensity of the nkx2.5 signal. Zebrafish embryos (1–2 cell stage) were injected with 16 ng of Std-MO (A,C), 16 ng of mog1 MO1 (B) or 16 ng of mog1 MO2 (D) and used for whole-mount in situ hybridization at 12 hpf with an antisense probe for nkx2.5. (G–J) Knockdown of mog1 expression reduces intensity of the gata4 signal. The embryos were injected and processed as in (A–D), but probed with an antisense probe for gata4. (MP) Knockdown of mog1 expression reduces expression of hand2. (SV) Knockdown of mog1 expression does not affect expression of gata5. (E,F,K,L,Q,R,W,X) Over-expression of zebrafish mog1 did not affect expression of cardiac transcriptional factors in the anterior lateral mesoderm at the 12 hpf stage. (AR1,S–X) Embryos are shown at a dorsal view with the anterior at the top. (M2R2) embryos are shown in a lateral view with the anterior on the left. The numbers in the upper right corner (e.g. 27/31 in A) describe the ratio of the number of embryos with the phenotype (e.g. 27) in the image over the number of total embryos analyzed (e.g. 31). Scale bar = 100 μM.

Expression Data
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 5-9 somites

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: 5-9 somites

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.